首页 抗体 一抗 一抗
PTEN (138G6) Rabbit mAb
PTEN (138G6) Rabbit mAb
1/3

PTEN (138G6) Rabbit mAb

分享
品牌： CST
pdf 下载产品说明书
pdf 下载SDS
用小程序，查商品更便捷
收藏
对比对比
咨询咨询
    分子量：
    54
    54
    反应种属：
    Human,Mouse,Rat,Monkey,
    Human,Mouse,Rat,Monkey,
    展开
    产品介绍
    产品信息
    抗原名称
    PTEN
    来源纯化
    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal sequence of human PTEN.
    宿主
    Rabbit
    简单描述
    Monoclonal Antibody for studying PTEN. Cited in 769 publications. Validated for WB, IP, IHC, IHC. Available in 3 sizes. Highly specific and rigorously validated in-house, PTEN (138G6) Rabbit Monoclonal Antibody (CST #9559) is ready to ship.
    商品描述

    Product Usage Information

    ApplicationDilution
    Western Blotting1:1000
    Immunoprecipitation1:100
    IHC Leica Bond1:100 - 1:400
    Immunohistochemistry (Paraffin)1:100 - 1:400
    分子量
    54
    研究领域
    癌症,细胞生物学,纤维化,代谢,神经科学
    应用
    反应种属
    Human,Mouse,Rat,Monkey,
    目标/特异性

    Specificity/Sensitivity

    PTEN (138G6) Rabbit mAb detects endogenous levels of total PTEN protein.

    Species Reactivity:

    Human, Mouse, Rat, Monkey

    敏感性
    Endogenous
    背景
    背景
    PTEN (phosphatase and tensin homologue deleted on chromosome ten), also referred to as MMAC (mutated in multiple advanced cancers) phosphatase, is a tumor suppressor implicated in a wide variety of human cancers (1). PTEN encodes a 403 amino acid polypeptide originally described as a dual-specificity protein phosphatase (2). The main substrates of PTEN are inositol phospholipids generated by the activation of the phosphoinositide 3-kinase (PI3K) (3). PTEN is a major negative regulator of the PI3K/Akt signaling pathway (1,4,5). PTEN possesses a carboxy-terminal, noncatalytic regulatory domain with three phosphorylation sites (Ser380, Thr382, and Thr383) that regulate PTEN stability and may affect its biological activity (6,7). PTEN regulates p53 protein levels and activity (8) and is involved in G protein-coupled signaling during chemotaxis (9,10). 1.Cantley, L.C. and Neel, B.G. (1999) Proc Natl Acad Sci USA 96, 4240-5. 2.Myers, M.P. et al. (1997) Proc Natl Acad Sci USA 94, 9052-7. 3.Myers, M.P. et al. (1998) Proc Natl Acad Sci USA 95, 13513-8. 4.Wan, X. and Helman, L.J. (2003) Oncogene 22, 8205-11. 5.Wu, X. et al. (1998) Proc Natl Acad Sci USA 95, 15587-91. 6.Vazquez, F. et al. (2000) Mol Cell Biol 20, 5010-8. 7.Torres, J. and Pulido, R. (2001) J Biol Chem 276, 993-8. 8.Freeman, D.J. et al. (2003) Cancer Cell 3, 117-30. 9.Funamoto, S. et al. (2002) Cell 109, 611-23. 10.Iijima, M. and Devreotes, P. (2002) Cell 109, 599-610.
    研究领域
    癌症,细胞生物学,纤维化,代谢,神经科学
    翻译后修饰
    Unmodified
    制备和贮存
    保存方式
    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product #98632.
    数据库链接
    Entrez-Gene ID
    5728
    UniProt ID
    P60484

    参考图片

    Immunohistochemical analysis of paraffin-embedded xenografts using PTEN (138G6) Rabbit mAb. DU145 (left) and A549 (middle) are PTEN positive cell lines, while U-87MG (right) is PTEN negative.

    免疫组化分析石蜡包埋的异种移植物，应用的抗体为PTEN (138G6) Rabbit mAb兔单抗。DU145 (左) 和 A549 (中) 是PTEN 阳性细胞系, 而 U-87MG (右) 是PTEN 阴性。

    Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft, using Phospho-Akt (Ser473) (736E11) Rabbit mAb (IHC Preferred) (#3787) (left) or PTEN (138G6) Rabbit mAb (right). MDA-MB-468 cells lack PTEN. Note the lack of PTEN staining in the Phospho-Akt positive cells.

    免疫组化分析石蜡包埋的MDA-MB-468 异种移植物，应用的抗体为Phospho-Akt (Ser473) (736E11) Rabbit mAb (IHC Preferred)兔单抗 (#3787) (左) 或者PTEN (138G6) Rabbit mAb兔单抗 (右). MDA-MB-468 细胞缺失PTEN。注意：在Phospho-Akt 阳性细胞中PTEN不能染色。

    Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using PTEN (138G6) Rabbit mAb in the presence of control peptide (left) or PTEN Blocking Peptide #1250 (right).

    免疫组织化学分析石蜡包埋的人结肠癌，所用抗体为PTEN (138G6) Rabbit mAb兔单抗，分别为对照肽段（左图）或者PTEN Blocking Peptide 封闭肽#1250 (右图）。

    Western blot analysis of extracts from A431, HeLa, 293, COS, PC12, NIH/3T3 and Mouse Brain cells, using PTEN (138G6) Rabbit mAb.

    Western blot 分析A431, HeLa, 293, COS, PC12, NIH/3T3 和小鼠脑细胞的提取物，所用抗体为PTEN (138G6) Rabbit mAb兔单抗。

    Immunohistochemical analysis of paraffin-embedded cell pellets demonstrating the specificity of PTEN (138G6) Rabbit mAb: DU145, HT-29 and MCF-7 (PTEN positive) and Jurkat, MDA-MB-468 and LNCaP (PTEN negative).

    免疫组化分析石蜡包埋的细胞团，能够显示出PTEN (138G6) Rabbit mAb兔单抗的特异性: DU145, HT-29 and MCF-7 (PTEN 阳性) 和 Jurkat, MDA-MB-468 and LNCaP (PTEN 阴性)。

    Immunohistochemical analysis of paraffin-embedded human lung carcinoma (left) and prostate carcinoma (right), using PTEN (138G6) Rabbit mAb. Note the stromal cell staining in the PTEN negative lung carcinoma, and the cancer cell staining in the PTEN positive prostate carcinoma.

    免疫组织化学分析石蜡包埋的人肺癌（左图）和前列腺癌（右图），所用抗体为PTEN (138G6) Rabbit mAb兔单抗。注意PTEN阴性的肺癌中基质细胞染色和PTEN阳性前列腺癌中癌细胞的染色。

    Immunohistochemical analysis of paraffin-embedded MDA-MB-468 xenograft using Phospho-Akt (Ser473) (D9E) Rabbit mAb #4060 (left) or PTEN (138G6) Rabbit mAb (right). Note the presence of P-Akt staining in the PTEN deficient MDA-MB-468 cells.

    免疫组织化学分析石蜡包埋的MDA-MB-468异种移植物，使用的抗体为Phospho-Akt (Ser473) (D9E) Rabbit mAb兔单抗 #4060 (左) 或者PTEN (138G6) Rabbit mAb兔单抗 (右)。注意：PTEN缺失的MDA-MB-468 细胞中，P-Akt 能够染色。

    Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® PTEN siRNA I (+), using PTEN Antibody #9552 and p42 MAPK (Erk2) Antibody #9108. PTEN Antibody confirms silencing of PTEN expression, while the p42 MAPK (Erk2) Antibody is used to control for loading and specificity of PTEN siRNA.

    Western blot 分析转染了100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) 或者SignalSilence® PTEN siRNA I (+)的HeLa细胞提取物，使用的抗体为PTEN Antibody #9552 和p42 MAPK (Erk2) Antibody #9108。PTEN 抗体证实了PTEN表达的沉默，而p42 MAPK (Erk2) Antibody是作为PTEN siRNA上样和特异性的对照。

    Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) or SignalSilence® PTEN siRNA II (+), using PTEN (138G6) Rabbit mAb and β-Actin (13E5) Rabbit mAb #4970. PTEN (138G6) Rabbit mAb confirms silencing of PTEN expression, while the β-Actin (13E5) Rabbit mAb is used to control for loading and specificity of PTEN siRNA.

    Western blot 分析转染了100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-) 或者SignalSilence® PTEN siRNA I (+)的HeLa细胞提取物，使用的抗体为PTEN (138G6) Rabbit mAb 和 β-Actin (13E5) Rabbit mAb兔单抗 #4970。 (138G6) Rabbit mAb兔单抗 证实了PTEN表达的沉默，而β-Actin (13E5) Rabbit mAb兔单抗 是作为PTEN siRNA上样和特异性的对照。

    声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。
    货号：
    9559L
    一键复制
    询价
    供应商现货
    20ul
    100ul
    300ul
    选择数量
    当前规格1件起购 
    配送至
    预计5-6个工作日送达，快递: 免运费，若需干冰额外收费
    询价 大包装询价