APC-R700 Mouse Anti-Human CD45(HI30)

品牌： BD Pharmingen

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参数

反应种属：

Human (QC Testing)

来源宿主：

Mouse IgG1, κ

产品介绍

产品信息

耦联标记

APC-R700

抗原名称

CD45

宿主

Mouse IgG1, κ

商品描述

The HI30 monoclonal antibody specifically binds to the 180, 190, 205, 220 kDa protein isoforms of CD45. CD45 is encoded by the PTPRC ( Protein tyrosine phosphatase receptor type C) gene. CD45, also known as the leukocyte common antigen (LCA), is a member of the protein tyrosine phosphatase (PTP) family. It is present on all human leukocytes including lymphocytes, monocytes, granulocytes, eosinophils, and thymocytes. CD45 is absent from circulating erythrocytes, platelets, or mature erythroid cells of bone marrow and non-hemopoietic tissues. This antibody was conjugated to BD Horizon APC-R700, which has been developed exclusively by BD Biosciences as a better alternative to Alexa Fluor® 700. APC-R700 excites and emits at similar wavelengths to Alexa Fluor® 700 yet exhibits significantly improved brightness. This dye can be excited by the red laser and detected with the same filter set as Alexa Fluor® (eg, 730/45-nm filter).

应用

反应种属

Human (QC Testing)

背景

别名

PTPRC; LCA; L-CA; Leukocyte Common Antigen; T200; GP180; LY5

制备和贮存

保存方式

Aqueous buffered solution containing BSA, protein stabilizer, glycerol and ≤0.09% sodium azide.

参考图片

Two-parameter flow cytometric analysis of CD45 expression on human peripheral blood leucocytes. Human whole blood was stained with either BD Horizon™ APC-R700 Mouse IgG1, κ Isotype Control (Cat. No. 564974; Left Plot) or BD Horizon APC-R700Mouse Anti-Human CD45 antibody (Cat. No. 566041/566042; Right Plot). The erythrocytes were lysed with BD FACS™ Lysing Solution (Cat. No. 349202). Two-parameter flow cytometric contour plots showing the correlated expression of CD45 (or Ig Isotype control staining) versus side-light scatter (SSC-A) signals were derived from gated events with the forward and side-light scatter characteristics of intact leucocyte populations. Flow cytometric analysis was performed using a BD LSRFortessa™ Cell Analyzer System.

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货号：

566042

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