Mouse Monoclonal CD45 Antibody (SPM570)

Unconjugated

Protein A or G purified

Mouse

Isolated neoplastic cells from T cell lymphoma

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-34804) Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

147 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

0.2 mg/ml

0.05% Sodium Azide

Monoclonal

PTPRC

Flow Cytometry 1-2 ug/million cellsImmunocytochemistry/ Immunofluorescence 1-2 ug/mlImmunohistochemistry Immunohistochemistry-Paraffin 1-2 ug/ml

HuSpecies Glossary

CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out). The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.