Quality-controlled near-haploid HAP1 wild-type cells

Why are HAP1 cells an ideal option for gene-edited cell line models?

Our HAP1 cell line database represents the synergy of two great systems: the amenability of HAP1 cells to gene editing and the precise gene editing capabilities of the CRISPR-Cas9 system. HAP1 is a near-haploid human cell line derived from the KBM-7 chronic myelogenous leukemia (CML) cell line. KBM-7 cells were derived from a male patient but lack a Y chromosome, thus containing a single X chromosome when in the haploid state. HAP1 cells tend to spontaneously diploidize over time. Additional monitoring of ploidy status and QC is necessary when fully haploid cultures are required. We offer several options for HAP1-related parental cell lines, summarized below. For more details on how the HAP1 cell line was created, their full genotype, and the additional QC recommended for assessment of ploidy status please see Beigl et al.

See our HAP1 publication list to see how the cell line has been applied for a wide range of biological processes and assays, such as DNA damage repair pathway, stress responses, disease modeling as well as antibody validation.

Parental Cell line (Catalog Number)	Fully Haploid Genetic Background	Duplication of Chr15	Extra Chr8	Screened for Ploidy Status
HAP1 (C631)
HAP1 screening ready (C859)
eHAP (C669)
KBM-7 (C628)

Parental cell line details

Human HAP1 Parental Cell Line (catalog number C631)

• Wild-type HAP1 cells
• Near haploid genetic background
• Duplication of a portion of Chr 15
• Not selected for ploidy status
• Will have haploid and diploid populations
• Parental cell line for our collection of HAP1 knockout cell lines
• Included with all KO cell lines as the parental control

Human HAP1 parental cell line - screening ready (catalog number C859)

• Wild-type HAP1 cells
• Near haploid genetic background
• Duplication of a portion of Chr 15
• Selected for ploidy status

Human eHAP cell line (catalog number C669)

• Fully-haploid engineered HAP1 (eHAP) cells
• Engineered to remove the fragment of chromosome 15 from chromosome 19
• No genes are duplicated in this cell line
• Has the Philadelphia chromosome
• Not screened for ploidy status
• Will have haploid and diploid populations

Limited use agreement

All cell lines engineered by Horizon Discovery are purchased under the terms of our Limited Use Label License.