首页 抗体 二抗 其他
R-Phycoerythrin-conjugated AffiniPure™ Fab Fragment Goat Anti-Human IgG, Fcγ Fragment Specific
R-Phycoerythrin-conjugated AffiniPure™ Fab Fragment Goat Anti-Human IgG, Fcγ Fragment Specific

R-Phycoerythrin-conjugated AffiniPure™ Fab Fragment Goat Anti-Human IgG, Fcγ Fragment Specific

分享
品牌： Jackson
pdf 下载产品说明书
pdf 下载SDS
用小程序，查商品更便捷
收藏
对比对比
咨询咨询
    反应种属：
    Human
    Human
    产品介绍
    产品信息
    抗原名称
    IgG, Fcγ Fragment

    Target: Human
    Host: Goat
    Antibody Format: Fab Fragment
    Specificity: IgG, Fcγ fragment specific
    Conjugate: R-Phycoerythrin
    Product Category: FabuLight™ - Affinity-Purified Fc Specific Fab Fragments
    Clonality: Polyclonal
    RRID: AB_2632442

    Information
    Based on immunoelectrophoresis and/or ELISA, the antibody reacts with the Fc portion of human IgG heavy chain but not with the Fab portion of human IgG. No antibody was detected against human IgM or IgA, or against non-immunoglobulin serum proteins. The antibody may cross-react with immunoglobulins from other species.

    Fab fragment antibodies are generated by papain digestion of whole IgG antibodies to remove the entire Fc portion, including the hinge region. These antibodies are monovalent, containing only a single antigen binding site. The molecular weight of Fab fragments is about 50 kDa.

    Usage
    Physical State: Freeze-dried solid

    Storage and Rehydration: Store freeze-dried solid at 2-8°C. Rehydrate with the indicated volume of dH2O (see product specification sheet) and centrifuge if not clear. Store at 2-8°C – do not freeze. Prepare working dilution on day of use.
    Expiration date: six months from date of rehydration. The expiration date may be extended if test results are acceptable for the intended use.


    Purity: The antibody was purified from antisera by a combination of papain digestion and immunoaffinity chromatography using antigens coupled to agarose beads. Fc fragments and whole IgG molecules have been removed.
    Buffer: 0.01M Sodium Phosphate, 0.25M NaCl, pH 7.6
    Stabilizer: 15 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
    Preservative: 0.05% Sodium Azide

    Suggested Working Concentration or Dilution Range:

    1:50 - 1:200 for sequential labeling applications.

    To complex with primary antibody in solution, use 3:1 weight ratio of R-PE:primary antibody (approximate 3:1 molar ratio of Fab:primary antibody). Vortex and incubate for 30 minutes at room temperature prior to use. Titrate complex to optimal dilution for assay.

    Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

    Conjugate

    R-Phycoerythrin

    Amax: 488 Emax: 580nm

    Phycoerythrin (R-PE) is among several kinds of light-harvesting phycobiliproteins found in red, blue-green, and cryptomonad algae. We offer R-PE, the form found in red macrophytic algae (seaweed). After phycobiliproteins are conjugated to secondary antibodies, there is little fluorescence quenching, which results in conjugates of high specific fluorescence compared with conventional fluorophore-antibody conjugates. R-PE can be excited by light over a wide range of the visible spectrum is highly water soluble, has a relatively low isoelectric point, and lacks potentially sticky carbohydrates.

    It should be noted that the relatively high molecular weight of R-PE may preclude its use in procedures requiring good penetration into cells and tissues. It is predominantly intended for surface labeling of cells for flow cytometry.

    组成成分
    藻红蛋白偶联山羊抗人免疫球蛋白G抗体;缓冲液(0.01M磷酸钠、0.25M氯化钠);稳定剂(1.5%牛血清白蛋白);防腐剂(0.05%叠氮化钠溶液)
    应用
    反应种属
    Human
    目标/特异性
    IgG, Fcγ fragment specific
    数据库链接
    研究资源识别码
    AB_2632442
    声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。
    货号：
    109-117-008
    一键复制
    询价
    0.5ml
    选择数量
    当前规格1件起购 
    配送至
    预计2-3周送达，快递: 免运费，若需干冰额外收费
    询价 大包装询价