Rabbit anti-HIF-1α Polyclonal Antibody

1/2

Rabbit anti-HIF-1α Polyclonal Antibody

品牌： Absin

下载产品说明书

用小程序，查商品更便捷

对比

咨询

参数

分子量：

Predicted molecular weight: 92kDa
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

反应种属：

Human, Mouse, Rat, Rabbit, Pig, Yak, Bovine, Cow, Guinea Pig

展开

产品介绍

产品信息

耦联标记

Unlabeled

纯化方式

affinity purified by Protein A

宿主

Rabbit

免疫原

KLH conjugated synthetic peptide derived from middle of human HIF-1 Alpha

同种型

IgG

分子量

克隆类别

Polyclonal Antibody

浓度

1mg/mL

应用

实验应用

WB: 1:500-2000, IHC-P: 1:100-500, IHC-F: 1:100-500, ICC: 1:100, IF: 1:100-500, FCM: 1ug/T, ELISA: 1:5000-10000

反应种属

Human, Mouse, Rat, Rabbit, Pig, Yak, Bovine, Cow, Guinea Pig

目标/特异性

HIF-1 Alpha

背景

别名

HIF-1 Alpha抗体

研究领域

肿瘤细胞生物神经生物学信号转导细胞凋亡

翻译后修饰

Unmodified

无

Expressed in most tissues with highest levels in kidney and heart. Overexpressed in the majority of common human cancers and their metastases, due to the presence of intratumoral hypoxia and as a result of mutations in genes encoding oncoproteins and tumor suppressors.

细胞定位

细胞核，细胞浆

制备和贮存

存储溶液

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存方式

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

参考图片

Sample:
1. A549 Cell (Human) Lysate at 40 ug
2. Hela Cell (Human) Lysate at 40 ug
3. Kidney (Mouse) Lysate at 40 ug
4. Heart (Mouse) Lysate at 40 ug
5. MCF-7 Cell (Human) Lysate at 40 ug
6. HL60 Cell (Human) Lysate at 40 ug
7. Ht1080 Cell (Human) Lysate at 40 ug
Primary: Anti- HIF-1 alpha (abs120168) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 92 kD
Observed band size: 120kD

Blank control (blue line): Hela (blue).
Primary antibody: Rabbit Anti- HIF-1 Alpha antibody
Dilution: 1μg /10^6 cells;
Isotype Control antibody: Rabbit IgG .
Secondary antibody: Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 80% methanol (5 min at -20℃) and then permeabilized with 0.1% PBS-Tween for 20 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

abs120168-50ul

一键复制

询价

50ul

100ul

选择数量

当前规格1件起购

配送至

预计1-2周送达，快递: 免运费，若需干冰额外收费

询价大包装询价