Rabbit anti-MCT1 Polyclonal Antibody

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Rabbit anti-MCT1 Polyclonal Antibody

品牌： Absin

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参数

分子量：

Predicted molecular weight: 55kDa
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

反应种属：

Human;Mouse;Rat;

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产品介绍

产品信息

耦联标记

Unlabeled

纯化方式

affinity purified by Protein A

宿主

Rabbit

免疫原

KLH conjugated synthetic peptide derived from human MCT1

同种型

IgG

分子量

克隆类别

Polyclonal Antibody

浓度

1mg/mL

应用

实验应用

WB: 1:500-2000, IHC-P: 1:100-500, IHC-F: 1:100-500, IF: 1:100-500, FCM: 1ug/T, ELISA: 1:5000-10000

反应种属

Human;Mouse;Rat;

目标/特异性

MCT1

背景

别名

MCT1抗体

研究领域

免疫学转运蛋白交换蛋白

翻译后修饰

Unmodified

无

Widely expressed in normal and in cancer cells.

细胞定位

细胞膜

制备和贮存

存储溶液

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存方式

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

参考图片

Sample:HepG2 (Human)cell Lysate at 40 ug
Primary: Anti-MCT1(abs120479)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 55kD
Observed band size: 55kD

Blank control (Black line): U937(Black).
Primary antibody: Rabbit Anti-MCT1/AF488 antibody
Dilution: 3μg /10^6 cells;
Isotype Control antibody: Rabbit IgG-AF488 .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.

Blank control: MCF7 Cells(blue).
Primary Antibody: Rabbit Anti-MCT1/AF647 Conjugated antibody, Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/FITC(orange) ,used under the same conditions.

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货号：

abs120479-50ul

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