Rabbit anti-GPX4 Polyclonal Antibody

1/3

Rabbit anti-GPX4 Polyclonal Antibody

品牌： Absin

下载产品说明书

用小程序，查商品更便捷

对比

咨询

参数

分子量：

Predicted molecular weight: 22kD
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

反应种属：

Human, Mouse, Rat

展开

产品介绍

产品信息

耦联标记

Unlabeled

纯化方式

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin .

宿主

Rabbit

免疫原

A synthesized peptide derived from human GPX4, corresponding to a region within C-terminal amino acids.

商品描述

Glutathione peroxidase catalyzes the reduction of hydrogen peroxide, organic hydroperoxide, and lipid peroxides by reduced glutathione and functions in the protection of cells against oxidative damage. Human plasma glutathione peroxidase has been shown to be a selenium-containing enzyme and the UGA codon is translated into a selenocysteine. Through alternative splicing and transcription initiation, rat produces proteins that localize to the nucleus, mitochondrion, and cytoplasm. In humans, experimental evidence for alternative splicing exists; alternative transcription initiation and the cleavage sites of the mitochondrial and nuclear transit peptides need to be experimentally verified. [provided by RefSeq, Jul 2008]

分子量

克隆类别

Polyclonal Antibody

浓度

1mg/ml

应用

实验应用

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500

反应种属

Human, Mouse, Rat

目标/特异性

GPX4 Antibody detects endogenous levels of total GPX4.

背景

别名

GPX4抗体

翻译后修饰

Unmodified

无

Present primarily in testis.

细胞定位

Cytosol;Extracellular region or secreted;Mitochondrion;Nucleus

制备和贮存

存储溶液

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.

保存方式

Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

参考图片

Rabbit anti-GPX4 Polyclonal Antibody at 1/100 staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.

Western blot analysis of extracts from various samples, using GPX4 antibody at 1/1000 dilution. Lane 1: Mouse testis. Lane 2: Ec304 cells(heat-shock treatment). Observed bands:20kD.

Rabbit anti-GPX4 Polyclonal Antibody staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary antibody(1:200) and mouse anti-beta tubulin antibody(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) antibody(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) antibody(Green) were used as the secondary antibody.
The nuclear counter stain is DAPI(blue).

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

abs136221-50ug

一键复制

询价

50ug

100ug

选择数量

当前规格1件起购

配送至

货期请咨询客服，快递: 免运费，若需干冰额外收费

询价大包装询价