Rabbit Polyclonal Laminin beta 1 Antibody

Unconjugated

Immunogen affinity purified

Rabbit

Laminin beta 1 Antibody was produced by repeated immunizations with a mixture of synthetic peptides corresponding to regions of adherence on laminin. (Uniprot: P07942)

This antibody has been prepared by immunoaffinity chromatography using immobilized human placental laminin followed by extensive cross-adsorption against human serum proteins and collagen and non-collagen extracellular matrix proteins to remove any unwanted specificitiesStore vial at 4C prior to opening. This product is stable at 4C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing.

Polyclonal

IgG purified

0.01% Sodium Azide

Polyclonal

LAMB1

Dot Blot ELISA 1:4000-1:8000Immunocytochemistry/ Immunofluorescence 0.25-2 ug/mlImmunohistochemistry 1:50-1:200Immunohistochemistry-Paraffin 1:10-1:500Immunoprecipitation 1:100Western Blot 1:500-1:2000

HuSpecies Glossary

Typically less than 1% cross-reactivity against other extracellular matrix proteins was detected by ELISA against purified standards. This antibody reacts with most mammalian Laminin beta 1s and has negligible cross-reactivity with Type I, II, III, IV, V or VI Collagens or Fibronectin. Non-specific cross-reaction of anti-Laminin beta 1 antibodies with other human serum proteins or non-Laminin beta 1 extracellular matrix proteins is negligible.

0.125M Sodium Borate, 75mM NaCl and 5mM EDTA, pH 8.0