rhDPPII, CF (10 ug)

品牌： R&D Systems

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产品信息

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3438-SE

Formulation	Supplied as a 0.2 μm filtered solution in Tris and NaCl.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 3 months, -20 to -70 °C under sterile conditions after opening.

Recombinant Human DPPII/QPP/DPP7 Protein, CF Summary

Product Specifications

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Lys-Pro-AMC (KP-AMC). The specific activity is >4,000 pmol/min/µg, as measured under the described conditions.

Source

Mouse myeloma cell line, NS0-derived human DPPII/QPP/DPP7 protein
Gly22-Leu492, with a C-terminal 10-His tag

Accession #

AAH11907

N-terminal Sequence
Analysis

Gly22 & Arg24

Predicted Molecular Mass

54 kDa

SDS-PAGE

64 kDa, reducing conditions

背景

别名

carboxytripeptidase,Dipeptidyl aminopeptidase II,dipeptidyl arylamidase II,dipeptidyl peptidase 2,Dipeptidyl peptidase 7,Dipeptidyl peptidase II,dipeptidylpeptidase 7,dipeptidyl-peptidase 7,dipeptidyl-peptidase II,DPP II,DPP2,DPP7,DPPII,EC 3.4.14.2,QPP,Quiescent cell proline dipeptidase,Dipeptidyl-peptidase II

背景

Background: DPPII/QPP/DPP7

Dipeptidyl-peptidase II (DPPII) is identical to quiescent cell proline dipeptidase (QPP) and dipeptidylpeptidase 7 (DPP7) (1, 2). It shares some substrate and cleavage specificity with DPPIV/CD26, DPP8, DPP9 and seprase/FAP (fibroblast activation protein), members of the S09 family of serine proteases. As prolyl proteases that cleave proteins and peptides after proline residues, these enzymes have high potential for drug discovery (3, 4). However, DPP7 is not a member of the S09 family, but a member of the S28 family that also includes lysosomal Pro‑X carboxypeptidase/prolylcarboxypeptidase/PRCP and thymus-specific serine peptidase/PRSS16 (2). The human DPP7 precursor consists of a signal peptide (aa 1‑21) and a mature chain (aa 22‑492). The purified rhDPP7 is active against Lys‑Pro-AMC and Lys‑Ala-AMC. Its activity against Lys‑Pro‑AMC is approximately 10-fold of that against Lys‑Ala‑AMC under otherwise identical conditions.

References

Underwood, R. et al. (1999) J. Biol. Chem. 274:34053.
Maes, M.B. et al. (2005) Biochem. J. 386:315.
Rosenblum, J.S. and J.W. Kozarich (2003) Curr. Opin. Chem. Biol. 7:496.
Lankas, G.R. et al. (2005) Diabetes 54:2988.

Long Name

Dipeptidyl-peptidase II

Entrez Gene IDs

29952 (Human); 83768 (Mouse); 83799 (Rat)

Alternate Names

carboxytripeptidase; Dipeptidyl aminopeptidase II; dipeptidyl arylamidase II; dipeptidyl peptidase 2; Dipeptidyl peptidase 7; Dipeptidyl peptidase II; dipeptidylpeptidase 7; dipeptidyl-peptidase 7; dipeptidyl-peptidase II; DPP II; DPP2; DPP7; DPPII; EC 3.4.14.2; QPP; Quiescent cell proline dipeptidase

研究领域

Related Research Areas

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货号：

3438-SE-010

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询价

10ug

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