Recombinant Human FGF-basic (154aa) Protein

SDS-PAGE: 1μg（R: reducing conditions, N: non-reducing conditions).

SEC-HPLC

EC50: 0.1-1 ng/ml, measured by the cell proliferation assay using Balb-3T3 cells.

Immobilized FGF-basic(154aa), Human (Cat. No. abs05144) at 1ug/mL (100uL/well) can bind FGF-R4/CD334 His Tag, Human (Cat. No. UA020009) with EC50 of 0.968ug/mL.

Immobilized FGF-basic(154aa), Human (Cat. No. abs05144) at 2.5ug/mL (100uL/well) can bind Human FGF-R1/CD331, His Tag (Cat. No. UA020008) with EC50 of 0.049-0.066ug/ mL.

Anti-His antibody Immobilized on CM5 Chip captured FGF-R1/CD331 His Tag, Human (Cat. No. UA020008), can bind FGF-basic(154aa), Human (Cat. No. abs05144) with an affinity constant of 0.33uM as determined in SPR assay.

FGF-basic(154aa), Human ，1ug（R: reducing conditions, N: non-reducing conditions).

Measured in a cell proliferation assay using Balb/c 3T3 mouse fibroblast cells.The EC50 for this effect is less than 0.1ng/mL.corresponding to a specific activity of > 1 x 107 units/mg.