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rhIFN-g (100 ug)
rhIFN-g (100 ug)
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rhIFN-g (100 ug)

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    产品介绍
    产品信息

    Scientific Data

    SEC-MALS View Larger

    Recombinant Human IFN-gamma (Catalog # 285-IF) has a molecular weight (MW) of 34.9 kDa as analyzed by SEC-MALS, suggesting that this protein is a homodimer.  MW may differ from predicted MW due to post-translational modifications (PTMs) present (i.e. Glycosylation).

    SDS-PAGE Recombinant Human IFN-gamma Protein SDS-PAGEView Larger

    1 μg/lane of Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.

    Bioactivity Recombinant Human IFN-gamma Protein BioactivityView Larger

    Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The activity is over 2-fold greater than the top competitor's IFN-gamma.

    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    285-IF

    FormulationLyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80 with BSA as a carrier protein.
    ReconstitutionReconstitute at 0.2 mg/mL in sterile, deionized water.  
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    285-IF/CF

    FormulationLyophilized from a 0.2 μm filtered solution in Sodium Succinate, Mannitol and Tween® 80.
    ReconstitutionReconstitute at 0.2 mg/mL in sterile, deionized water. 
    ShippingThe product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    Recombinant Human IFN-gamma Protein Summary

    Product Specifications

    Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
    Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
    Activity
    Measured in anti-viral assays using HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 0.15-0.75 ng/mL.
    Source
    E. coli-derived human IFN-gamma protein
    Gln24-Gln166 with an N-terminal Met
    Accession #
    CAA31639
    N-terminal Sequence
    Analysis
    Met
    Predicted Molecular Mass
    16.9 kDa
    SDS-PAGE
    17 kDa, reducing conditions
    背景
    别名
    IFG,IFI,IFNG,IFNgamma,IFN-gamma,Immune interferon,interferon gamma,interferon, gamma,Interferon gamma
    背景

    Background: IFN-gamma

    Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature human IFN-gamma exists as a non-covalently linked homodimer of 20-25 kDa variably glycosylated subunits (3). It shares 90% amino acid (aa) sequence identity with rhesus IFN-gamma, 59%‑64% with bovine, canine, equine, feline, and porcine IFN‑ gamma, and 37%‑43% with cotton rat, mouse, and rat IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI ( alpha subunits) which then interact with IFN-gamma RII ( beta  subunits) to form the functional receptor complex of two alpha and two beta  subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN-gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, up‑regulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

    References
    1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
    2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
    3. Gray, P.W. and D.V. Goeddel (1982) Nature 298:859.
    4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
    5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
    6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
    7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
    8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
    9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
    Long Name
    Interferon gamma
    Entrez Gene IDs
    3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
    Alternate Names
    IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 0.2 mg/mL in sterile, deionized water.  

    参考图片

    1 μg/lane of Recombinant Human IFN-gamma was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 17 kDa.

    Recombinant Human IFN-gamma (Catalog # 285‑IF) demonstrates anti-viral activity in HeLa human cervical epithelial carcinoma cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is typically 0.15‑0.75 ng/mL.

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    货号：
    285-IF-100
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    询价
    100ug
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