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rhProstasin/Prss8, C (10 ug)
rhProstasin/Prss8, C (10 ug)

rhProstasin/Prss8, C (10 ug)

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品牌： R&D Systems
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    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    4599-SE

    FormulationSupplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
    ShippingThe product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
    Stability & Storage:Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 6 months from date of receipt, -20 to -70 °C as supplied.
    • 3 months, -20 to -70 °C under sterile conditions after opening.

    Recombinant Human Prostasin/Prss8 Protein, CF Summary

    Product Specifications

    Purity
    >95%, by SDS-PAGE under reducing conditions and visualized by silver stain
    Endotoxin Level
    <1.0 EU per 1 μg of the protein by the LAL method.
    Activity
    Measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC (Catalog # ES014). The specific activity is >10 pmol/min/µg, as measured under the described conditions.
    Source
    Chinese Hamster Ovary cell line, CHO-derived human Prostasin/Prss8 protein
    Ala33-Gly319, with a C-terminal 10-His tag
    The pro form was activated and further purified.
    Accession #
    NP_002764
    N-terminal Sequence
    Analysis
    Ala33 & Ile45
    Structure / Form
    Active
    Predicted Molecular Mass
    32 kDa (Pro) & 31 kDa (Mature)
    SDS-PAGE
    39 kDa, reducing conditions
    背景
    别名
    CAP1,channel-activating protease 1,EC 3.4.21,EC 3.4.21.-,EC 3.4.21.120,Prostasin,protease, serine, 8,Prss8,Serine protease 8
    背景

    Background: Prostasin/Prss8

    Prostasin, also known as channel activating protease 1, is a serine protease with trypsin-like substrate specificity (1, 2). The preproenzyme possesses a C-terminal membrane-spanning domain that can be proteolytically processed to generate a secreted form of the enzyme. The secreted form of Prostasin has been purified from seminal fluid (2). Prostasin is highly expressed in the prostate gland, and is expressed at lower levels in the lung, kidney, liver, salivary gland, and pancreas (3). The peptidase activity of Prostasin is involved in the regulation of epithelial sodium channels (4, 5).

    References
    1. Vuagniaux, G. et al. (2000) J. Am. Soc. Nephrol. 11:828.
    2. Yu, J.X. et al. (1994) J. Biol. Chem. 269:18843.
    3. Yu, J.X. et al. (1995) J. Biol. Chem. 270:13483.
    4. Caldwell, R.A. et al. (2004) Am. J. Physiol. Cell Physiol. 286:C194.
    5. Tong, Z. et al. (2004) Am. J. Physiol. Lung Cell Mol. Physiol. 287:L928.
    Entrez Gene IDs
    5652 (Human); 76560 (Mouse)
    Alternate Names
    CAP1; channel-activating protease 1; EC 3.4.21; EC 3.4.21.-; EC 3.4.21.120; Prostasin; protease, serine, 8; Prss8; Serine protease 8
    研究领域
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    货号：
    4599-SE-010
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    询价
    10ug
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