Recombinant Human TNF-α Protein

2μg (N: non-reducing conditions, R: reducing conditions).

EC50 < 0.1ng/ml, measured in a cytotoxicity assay using L-929.

EC50 < 0.1ng/mL, measured in a cytotoxicity assay using L-929.

Immobilized TNF-α, Human (Cat. No. abs05092) at 10ug/mL (100uL/well) can bind TNFR-1/CD120a His Tag, Human (Cat. No. UA040028) with EC50 of 0.167 ug/mL.

Anti-His antibody Immobilized on CM5 Chip captured TNFR-1/CD120a His Tag, Human (Cat. No. UA040028), can bind TNF-α, Human (Cat. No. abs05092) with an affinity constant of 0.106nM as determined in SPR assay.

TNF-α, Human，2ug on SDS-PAGE under Non-reducing and reducing condition. The purity is greater than 95%.

Anti-His antibody Immobilized on CM5 Chip captured TNFR-2/CD120b His Tag, Human (Cat. No. UA010138), can bind TNF-α, Human (Cat. No. abs05092) with an affinity constant of 1.53 nM as determined in SPR assay.

Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The EC50 for this effect is less than 10pg/mL.