Recombinant SUMO Protease, Yeast

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Recombinant SUMO Protease, Yeast

品牌： UA BIOSCIENCE

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参数

分子量：

27 kDa (Reducing)

纯度：

＞95% by SDS-PAGE and RP-HPLC

产品介绍

产品信息

耦联标记

Unconjugated

分子量

27 kDa (Reducing)

生物活性

10U/μl

酶活定义

One unit of SUMO Protease cleaves ≥85% of 2 μg control substrate in 1 h at 30°C.

纯度

＞95% by SDS-PAGE and RP-HPLC

内毒素水平

<1EU/μg

性状

Liquid

表达系统

E.coli

缓冲体系

20mM PB, pH7.4, 200mM NaCl, 0.1% NP-40, 0.5mM DTT, 50%(v/v) Glycerol

组合货号

1. SUMO Protease;

2. 10X SUMO Protease Buffer + Salt: 500 mM PB, pH 7.4, 2% Igepal (NP-40), 1.5 M NaCl, 10 mM DTT;

3. 10X SUMO Protease Buffer – Salt: 500 mM PB, pH 7.4, 2% Igepal (NP-40), 10 mM DTT;

标签

No Tag

种属

Yeast

电泳JSON

[{"imgUrl":"https://univ-njua.oss-cn-shanghai.aliyuncs.com/prod/202208/43561b55376b41a48af61881820f500a.jpg","description":"1μg (N: non-reducing condition, R: reducing condition)."}]

背景

Saccharomyces cerevisiae-derived Small ubiquitin-like modifier (SUMO, Smt3) is commonly used as a protein fusion domain to facilitate expression and purification of recombinant proteins, and a Saccharomyces cerevisiae-derived SUMO-specific protease(Ulp1) is then used to remove SUMO tag from these proteins in a ‘scarless’ manner. SUMO Protease cleaves in a highly specific manner, recognizing the tertiary structure of the SUMO tag, rather than an amino acid sequence, and hydrolyzes the peptide bond in the x-Gly-Gly-x sequence after the Gly-Gly bond at the C-terminus of the SUMO tag. The SUMO Protease cleavage proteins over wide ranges of temperature (4℃-30℃), ionic strengths(0-400 mM NaCl) and pH(7.0-9.0), and easily removed from the cleavage reaction by Immobilized Metal Affinity chromatography (IMAC).

制备和贮存

保存方式

· 12 months from date of receipt, -20 to -70 °C as supplied.
· 6 months, -20 to -70 °C under sterile conditions after reconstitution.
· 1 week, 2 to 8 °C under sterile conditions after reconstitution.
· Please avoid repeated freeze-thaw cycles.

参考图片

The substrate of 2 μg fusion protein was digested by SUMO Protease at 30 ℃ for 1 hour.

The addition amount of SUMO Protease were 0U, 0.25U, 0.5U, 0.75U and 1U, respectively.

The substrate is a fusion protein with a molecular weight of 20 KD and digested completely into two bands.

1μg (N: non-reducing condition, R: reducing condition).

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货号：

UA070004-1kU

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