S-RMab® CK-Pan Recombinant Rabbit mAb (SDT-P001)

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S-RMab® CK-Pan Recombinant Rabbit mAb (SDT-P001)

品牌： STARTER

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参数

反应种属：

来源宿主：

Rabbit

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产品介绍

产品信息

纯化方式

Protein A

抗原名称

CK-Pan

宿主

Rabbit

免疫原

N/A

克隆号

SDT-P001

浓度

Lot specific* (generally 0.3 to 0.5 mg/ml)*

性状

Liquid

缓冲体系

PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300

产品类型

Rabbit mAb

应用

实验应用

WB、IHC-P、IF

反应种属

稀释度

IHC-P

1:500-1:2000
WB

1:1000
IF

1:500

背景

别名

Pan Cytokeratin, Pan CK

背景

CK (Pan) mainly marks keratinized epithelium, stratified squamous epithelium, stratified epithelium, proliferative keratinocytes, and monolayer epithelium, and is used in squamous cell carcinoma (including Spindle variant), various adenocarcinoma (including adrenal carcinoma and hepatocellular carcinoma), transitional cell carcinoma, small cell carcinoma, malignant mesothelioma, germ cell tumor (excluding spermatogonium Tumor, partial synovial sarcoma, and leiomyosarcoma can be positively labeled. Pathology is mainly applied to the differential diagnosis of epithelial tumors and non epithelial tumors.

细胞定位

Cytoplasm

制备和贮存

保存方式

12 months from date of receipt / reconstitution, -20 °C as supplied

参考图片

IHC shows positive staining in paraffin-embedded human colon. Anti-CK-Pan antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human hepatocellular carcinoma. Anti-CK-Pan antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung adenocarcinoma. Anti-CK-Pan antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human lung squamous cell carcinoma. Anti-CK-Pan antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human pancreatic carcinoma. Anti-CK-Pan antibody was used at 1/2000 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

WB result of CK-Pan Rabbit mAb

Primary antibody: CK-Pan Rabbit mAb at 1/1000 dilution
Lane 1: HeLa whole cell lysate 20 µg
Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution
Predicted MW: 40~70 kDa
Observed MW: 40~55 kDa
Exposure time: 13s

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货号：

S0B2118-10μl

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