SIE consensus oligonucleotide

consensus binding site (Sis-inducible element) for Sis-inducible factor; supplied as 500 ng double-stranded DNA; sc-2535also available as mutant oligonucleotide with a "TTC"→"CCA" substitution in the DNA binding region; sc-25365′-GTG CAT TTC CCG TAA ATC TTG TCT ACA-3′also available as an agarose conjugate for use in purifying or enriching specific transcription factors; 15 µg in 0.25 ml packed beads; sufficient for 10 assays; use 25 µl beads per 0.5 - 1 mg total cell lysate protein, sc-2501 AC 查看产品引用文献(36) Electrophoretic mobility shift assays (EMSAs), also known as gel shift assays, provide a relatively straightforward and sensitive method for studying binding interactions between transcription factors and consensus DNA binding elements. For such studies, DNA probes are provided as double stranded oligonucleotides designed with 5’ OH blunt ends to facilitate labeling to high specific activity with polynucleotide kinase. These are constructed both with specific DNA binding consensus sequences for various transcription factors and as control or “mutant” probes in which one or more nucleotides mapping within the consensus binding site has been substituted.