



Product Usage Information
CST recommends transfection with 100 nM c-Myc siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.






参考图片
Western blot analysis of extracts from HeLa cells following mock transfection, transfection with nonspecific (control) siRNA or transfection with SignalSilence® c-Myc siRNA I, using c-Myc Antibody #9402 and p42 MAP Kinase (Erk2) Antibody #9108. The c-Myc antibody confirms silencing of c-Myc expression and the p42 MAPK antibody is used to control for loading and specificity of c-Myc siRNA.
Western blot 检测HeLa细胞提取物,分别用空质粒、非特异性Control siRNA或SignalSilence® c-Myc siRNA I转染;使用c-Myc Antibody #9402或p42 MAP Kinase (Erk2) Antibody #9108检测。c-Myc antibody确定c-Myc表达的沉默,p42 MAPK antibody作为内参对照使用。
Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency.
荧光检测SignalSilence® Control siRNA (Fluorescein Conjugate) #6201转染24小时后的HeLa细胞,观察到了近100%的转染效率。