SignalSilence ®  c-Myc siRNA I
1/2

SignalSilence ® c-Myc siRNA I

分享
品牌: CST
pdf 下载产品说明书
用小程序,查商品更便捷
收藏
对比对比
咨询咨询
    反应种属:
    Human
    Human
    产品介绍
    产品信息
    简单描述
    siRNA for studying Myc in the research area.
    商品描述

    Product Usage Information

    CST recommends transfection with 100 nM c-Myc siRNA I 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.

    研究领域
    细胞生物学,纤维化,神经科学
    应用
    反应种属
    Human
    背景
    背景
    Members of the Myc/Max/Mad network function as transcriptional regulators with roles in various aspects of cell behavior, including proliferation, differentiation, and apoptosis (1). These proteins share a common basic-helix-loop-helix leucine zipper (bHLH-ZIP) motif required for dimerization and DNA-binding. Max was originally discovered based on its ability to associate with c-Myc and found to be required for the ability of Myc to bind DNA and activate transcription (2). Subsequently, Max has been viewed as a central component of the transcriptional network, forming homodimers as well as heterodimers with other members of the Myc and Mad families (1). The association between Max and either Myc or Mad can have opposing effects on transcriptional regulation and cell behavior (1). The Mad family consists of four related proteins; Mad1, Mad2 (Mxi1), Mad3, and Mad4, and the more distantly related members of the bHLH-ZIP family, Mnt and Mga. Like Myc, the Mad proteins are tightly regulated with short half-lives. In general, Mad family members interfere with Myc-mediated processes, such as proliferation, transformation, and prevention of apoptosis by inhibiting transcription (3,4). 1.Baudino, T.A. and Cleveland, J.L. (2001) Mol Cell Biol 21, 691-702. 2.Blackwood, E.M. and Eisenman, R.N. (1991) Science 251, 1211-7. 3.Henriksson, M. and Lüscher, B. (1996) Adv Cancer Res 68, 109-82. 4.Grandori, C. et al. (2000) Annu Rev Cell Dev Biol 16, 653-99.
    研究领域
    细胞生物学,纤维化,神经科学
    制备和贮存
    保存方式
    SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.
    数据库链接
    Entrez-Gene ID
    4609
    UniProt ID
    P01106

    参考图片

    Western blot analysis of extracts from HeLa cells following mock transfection, transfection with nonspecific (control) siRNA or transfection with SignalSilence® c-Myc siRNA I, using c-Myc Antibody #9402 and p42 MAP Kinase (Erk2) Antibody #9108. The c-Myc antibody confirms silencing of c-Myc expression and the p42 MAPK antibody is used to control for loading and specificity of c-Myc siRNA.

    Western blot 检测HeLa细胞提取物,分别用空质粒、非特异性Control siRNA或SignalSilence® c-Myc siRNA I转染;使用c-Myc Antibody #9402或p42 MAP Kinase (Erk2) Antibody #9108检测。c-Myc antibody确定c-Myc表达的沉默,p42 MAPK antibody作为内参对照使用。

    Fluorescent detection of SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 in living HeLa cells 24 hours post-transfection, demonstrating nearly 100% transfection efficiency.

    荧光检测SignalSilence® Control siRNA (Fluorescein Conjugate) #6201转染24小时后的HeLa细胞,观察到了近100%的转染效率。

    声明 :本官网所有报价均为常温或者蓝冰运输价格,如有产品需要干冰运输,需另外加收干冰运输费。
    货号:
    6341S
    一键复制
    询价
    300ul
    选择数量
    当前规格1件起购 
    配送至
    预计3-4周送达,快递: 免运费,若需干冰额外收费