SignalSilence ® Control siRNA (Unconjugated)

品牌： CST

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产品介绍

产品信息

耦联标记

Unconjugated

简单描述

siRNA for studying Control siRNA (Fluorescein Conjugate) in the research area.

商品描述

Product Usage Information

CST recommends transfection with 100 nM Control siRNA II 48 to 72 hours prior to cell lysis. For transfection procedure, follow protocol provided by the transfection reagent manufacturer. Please feel free to contact CST with any questions on use.Each vial contains the equivalent of 50 transfections, which corresponds to a final siRNA concentration of 100 nM per transfection in a 24-well plate with a total volume of 300 μl per well.

背景

制备和贮存

保存方式

SignalSilence® siRNA is supplied in RNAse-free water. Aliquot and store at -20ºC.

参考图片

Western blot analysis of extracts from HeLa cells 48 hours following transfection with 100 nM non-targeted SignalSilence® Control siRNA (Unconjugated). Protein expression levels are measured using Akt (pan) (C67E7) Rabbit mAb #4691 (left), β-Actin (13E5) Rabbit mAb #4970 (middle) or p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (right). Western blot分析用100 nM non-targeted SignalSilence ® Control siRNA (Unconjugated) 转染48小时的HeLa细胞提取物。分别用 Akt (pan) (C67E7) Rabbit mAb #4691 (左), β-Actin (13E5) Rabbit mAb #4970 (中) or p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb #4695 (右)检测蛋白的表达水平。

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货号：

6568S

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150ul

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