首页 抗体 一抗 流式抗体
BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Sox9
BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Sox9
1/2

BD Pharmingen™ Alexa Fluor® 647 Mouse Anti-Sox9

分享
品牌： BD Pharmingen
pdf 下载产品说明书
pdf 下载SDS
用小程序，查商品更便捷
收藏
对比对比
咨询咨询
    反应种属：
    Human (QC Testing), Rat (Tested in Development)
    Human (QC Testing), Rat (Tested in Development)
    来源宿主：
    Mouse IgG1, κ
    Mouse IgG1, κ
    展开
    产品介绍
    产品信息
    抗原名称
    Sox9
    宿主
    Mouse IgG1, κ
    免疫原
    Human Sox9 Recombinant Protein
    简单描述
    The T32-668 monoclonal antibody specifically binds to Sox9, a member of the SRY-related HMG-box (SOX) family of transcription factors. Sox9 plays a key role in skeletal development and sex determination. Mutation of Sox9 has been associated with the human skeletal dysmorphology syndrome campomelic dysplasia, which is often characterized by both skeletal abnormalities and sex reversal. Expression of Sox9 has been observed in a variety of tissues and organs, including cartilage, testis, central neural system, lung, pancreas, intestinal stem cells, and heart.
    商品描述
    T32-668 The T32-668 monoclonal antibody specifically binds to Sox9, a member of the SRY-related HMG-box (SOX) family of transcription factors. Sox9 plays a key role in skeletal development and sex determination. Mutation of Sox9 has been associated with the human skeletal dysmorphology syndrome campomelic dysplasia, which is often characterized by both skeletal abnormalities and sex reversal. Expression of Sox9 has been observed in a variety of tissues and organs, including cartilage, testis, central neural system, lung, pancreas, intestinal stem cells, and heart.
    同种型
    Mouse IgG1, κ
    克隆号
    克隆 T32-668 (RUO)
    浓度
    0.2 mg/ml
    产品详情
    Alexa Fluor™ 647
    Alexa Fluor™ 647 Dye is part of the BD red family of dyes. This is a small organic fluorochrome with an excitation maximum (Ex Max) at 653-nm and an emission maximum (Em Max) at 669-nm. Alexa Fluor 647 is designed to be excited by the Red laser (627-640 nm) and detected using an optical filter centered near 520-nm (e.g., a 660/20 nm bandpass filter). Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.
    应用
    实验应用
    Intracellular staining (flow cytometry) (Routinely Tested), Bioimaging, Immunofluorescence (Not Recommended)
    反应种属
    Human (QC Testing), Rat (Tested in Development)
    目标/特异性
    Sox9
    背景
    别名
    SOX-9, SOX9
    制备和贮存
    存储溶液
    Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
    保存方式
    Aqueous buffered solution containing protein stabilizer and ≤0.09% sodium azide.
    文献
    文献
    研发参考(5) 1. Furuyama K, Kawaguchi Y, Akiyama H, et al. Continuous cell supply from a Sox9-expressing progenitor zone in adult liver, exocrine pancreas and intestine. Nat Genet. 2011; 43(1):34-41. (Biology). 2. Guo W, Keckesova Z, Donaher JL, et al. Slug and Sox9 cooperatively determine the mammary stem cell state. Cell. 2012; 148(5):1015-1028. (Biology). 3. Kent J, Wheatley SC, Andrews JE, Sinclair AH, Koopman P. A male-specific role for SOX9 in vertebrate sex determination. Development. 1996; 122(9):2813-2822. (Biology). 4. Stolt CC, Lommes P, Sock E, Chaboissier MC, Schedl A, Wegner M. The Sox9 transcription factor determines glial fate choice in the developing spinal cord. Genes Dev. 2003; 17(13):1677-1689. (Biology). 5. Wright E, Hargrave MR, Christiansen J, et al. The Sry-related gene Sox9 is expressed during chondrogenesis in mouse embryos. Nat Genet. 1995; 9(1):15-20. (Biology).

    参考图片

    Flow cytometric analysis of SOX9 expression in human hepatocellular carcinoma and embryonic stem cell lines. Hep G2 cells (ATCC HB-8065, Left Panel) and H9 cells (WiCell, Madison, WI; Right Panel) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were stained with either Alexa Fluor® 647 Mouse IgG1 κ Isotype Control (Cat. No.557732; dashed line histogram) or Alexa Fluor® 647 Mouse Anti-Sox9 (Cat. No. 565493; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact Hep G2 or H9 cells, respectively. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.

    Flow cytometric analysis of SOX9 expression in human hepatocellular carcinoma and embryonic stem cell lines. Hep G2 cells (ATCC HB-8065, Left Panel) and H9 cells (WiCell, Madison, WI; Right Panel) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655), and permeabilized with BD Phosflow™ Perm Buffer III (Cat. No. 558050). The cells were stained with either Alexa Fluor® 647 Mouse IgG1 κ Isotype Control (Cat. No.557732; dashed line histogram) or Alexa Fluor® 647 Mouse Anti-Sox9 (Cat. No. 565493; solid line histogram). The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact Hep G2 or H9 cells, respectively. Flow cytometric analysis was performed using a BD FACSCanto™ II Flow Cytometer System.

    声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。
    货号：
    565493
    一键复制
    询价
    供应商现货
    50ug
    选择数量
    当前规格1件起购 
    配送至
    预计5-6个工作日送达，快递: 免运费，若需干冰额外收费
    询价 大包装询价