Streptavidin (Sepharose ® Bead Conjugate)

品牌： CST

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耦联标记

Sepharose Bead

来源纯化

Streptavidin is expressed in

简单描述

Miscellaneous for studying Streptavidin in the research area.

商品描述

Product Usage Information

Add 10 μl of well-vortexed beads to 200 μl of cell lysate at 1 mg/ml pre-incubated with biotinylated primary antibody. See protocol for more details.

应用

目标/特异性

Specificity/Sensitivity

Streptavidin has a remarkably high affinity for its natural ligand, biotin. The complex and irregular structure of the biotin-binding site makes it highly optimized for biotin binding and confers great specificity to the streptavidin-biotin complexes (4).

背景

Streptavidin is a 53,000 dalton tetrameric protein purified from the bacterium Streptomyces avidinii (3). Each subunit binds to biotin with extremely high affinity. Because of its strong non-covalent interaction with biotin, streptavidin can be used to isolate biotinylated proteins (1,2). 1.Updyke, T.V. and Nicolson, G.L. (1984) J Immunol Methods 73, 83-95. 2.Buckie, J.W. and Cook, G.M. (1986) Anal Biochem 156, 463-72. 3.Chaiet, L. and Wolf, F.J. (1964) Arch Biochem Biophys 106, 1-5. 4.Reznik, G.O. et al. (1998) Proc Natl Acad Sci U S A 95, 13525-30.

制备和贮存

保存方式

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA, 50% glycerol. Store at –20°C. Do not aliquot the antibodies.

参考图片

Immunoprecipitation of extracts from COS cells transfected with Myc-Chorionic Somatomammotropin Hormone-Like 1 (CSL) protein using Myc-Tag (9B11) Mouse mAb (Biotinylated) #2084 and Immobilized Streptavidin (Bead Conjugate) (Lane 1) shows immunocomplexes pulled down using Streptavidin (Sepharose Bead Conjugate). Lysate and beads alone are shown in lane 2 indicating the specificity of the streptavidin beads. Western blotting was performed using Myc-Tag (9B11) Mouse mAb (HRP Conjugate) #2040.免疫沉淀分析Myc-绒毛膜滋养层激素1(CSL) 蛋白转染的COS细胞的提取物。所用抗体为Myc-Tag (9B11) Mouse mAb (生物素化) #2084 和链霉素亲和素(微球结合) (泳道 1) ，表明链霉素亲和素(微球结合) 已经将免疫复合体沉淀。泳道2 是单独的细胞提取物和微球提示了链霉素亲和素微球的特异性。用Myc-Tag (9B11) Mouse mAb (HRP Conjugate) #2040进行western blotting分析。

Immunoprecipitation of extracts from Jurkat cells using Phospho-Akt (Thr308) (244F9) Rabbit mAb (Biotinylated) #3454 and Streptavidin (Sepharose Bead Conjugate) (Lane 1). Lysate and beads alone are shown in lane 2 indicating the specificity of the streptavidin beads. Western blotting was performed using Phospho-Akt (Thr308) (L32A4) Mouse mAb #5106.免疫沉淀分析Jurkat细胞的提取物，所用抗体为Phospho-Akt (Thr308) (244F9) Rabbit mAb (生物素化) #3454 和链霉素亲和素(微球结合) (泳道 1) 。泳道2 是单独的细胞提取物和微球提示了链霉素亲和素微球的特异性。用Phospho-Akt (Thr308) (L32A4) Mouse mAb #5106进行western blotting分析。

Immunoprecipitation of extracts from NIH/3T3 cells using PTEN (138G6) Rabbit mAb (Biotinylated) #9583 and Streptavidin (Sepharose Bead Conjugate) (Lane 1). Lysate and beads alone are shown in lane 2 indicating the specificity of the streptavidin beads. Western blotting was performed using PTEN (26H9) Mouse mAb #9556.免疫沉淀分析NIH/3T3细胞，所用抗体为PTEN (138G6) Rabbit mAb (Biotinylated) #9583 and链霉素亲和素(琼脂糖微珠结合) (泳道1)。泳道2 是单独的细胞提取物和微球提示了链霉素亲和素微球的特异性。用PTEN (26H9) Mouse mAb #9556进行western blotting分析。

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货号：

3419S

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400ul

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