视频素材来源于YouTube https://www.youtube.com/watch?v=PVSSgScb8v4
版权归原作者所有
person相关系数:相关系数的绝对值越大，相关性越强;相关系数越接近于1或-1，相关度越强，相关系数越接近于0，相关度越弱。为了评估组织与类器官的一致性，采用配对法计算了组织和类器官之间的person相关系数，然后对相关性矩阵进行z评分。
实验步骤
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类器官RNA的提取和继续培养
从一部分中直接分离RNA(RNeasy Mini Kit; Qiagen); 另一部分培养1周，然后分离RNA。我们根据制造商的说明制备了标记的反义RNA(Agilent Technologies)
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使用Smartseq2方法分析
使用Smartseq2方法分析
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使用Illumina HiSeq进行RNA测序
使用Illumina HiSeq进行RNA测序
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参考文献
P.S.以上实验步骤均来自文献总结，仅供参考
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