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实验步骤
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⼩⿏Th细胞分化条件
产品名称 Tho Th1 Th2 Th17 Treg anti-CD3
可溶性抗体
5μg/ml 5μg/ml 5μg/ml 5μg/ml 5μg/ml anti-CD28
可溶性抗体
2μg/ml 2μg/ml 2μg/ml 2μg/ml 2μg/ml IL-2
重组
细胞因子
4ng/ml 4ng/ml 4ng/ml IL-2
重组
细胞因子
4ng/ml 4ng/ml 4ng/ml IL-4
重组
细胞因子
100ng/ml IL-4
重组
细胞因子
100ng/ml IL-12
重组
细胞因子
10ng/ml IL-12
重组
细胞因子
10ng/ml TGF-β
重组
细胞因子
5ng/ml 1ng/ml TGF-β
重组
细胞因子
5ng/ml 1ng/ml IL-6
重组
细胞因子
100ng/ml IL-6
重组
细胞因子
100ng/ml anti-IL-4
Blocking
阻断抗体
10μg/ml 10μg/ml anti-IFN-γ
Blocking
阻断抗体
10μg/ml 10μg/ml anti-IL-12
Blocking
阻断抗体
10μg/ml -
Mouse脾脏 Th1/Th2/Th17胞内流式检测案例流式图
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Flow cytometric analysis of MouseTh1/Th2/Th17 phenotypingkit.The staining pattern ofIFN-g,lL-17A and lL-4 on resting spleen cells(leftcolumn),PMA/lonomycin stimulated spleencells(middlecolumn)and polarized Th17 cells (rightcolumn) are shown.The top three panels show hestaining oflL-17A vs.IFN-y and bottom three panelsshow the staining oflL-17A vs IL-4.Th17 cells were generated from mouse spleen cells for a 14 days period under polarizing condition described by Chen Dong etal(seereferencesbelow).Dead cells appearon the diagonal.Dot plotanalyses are derived fromgated CD4+ cell populations.Flow cytometry wasperformed on a BD FACS Calibur TM.System.