Rabbit anti-GPR91 Polyclonal Antibody

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Rabbit anti-GPR91 Polyclonal Antibody

品牌： Absin

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参数

分子量：

Predicted molecular weight: 39kDa
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

反应种属：

Human;Mouse;

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产品介绍

产品信息

耦联标记

Unlabeled

纯化方式

affinity purified by Protein A

宿主

Rabbit

免疫原

KLH conjugated synthetic peptide derived from human GPR91

同种型

IgG

分子量

克隆类别

Polyclonal Antibody

浓度

1mg/mL

应用

实验应用

WB: 1:500-2000, IHC-F: 1:100-500, IHC-P: 1:100-500, IF: 1:100-500

反应种属

Human;Mouse;

目标/特异性

GPR91

背景

别名

GPR91抗体

研究领域

细胞生物免疫学信号转导 G蛋白偶联受体 G蛋白信号

翻译后修饰

Unmodified

无

Expressed specifically in kidney.

细胞定位

细胞膜

制备和贮存

存储溶液

0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.

保存方式

Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

参考图片

Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;
Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated secondary primary antibody 1:600, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

Tissue/cell: human breast cancer; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;
Incubation: Anti-GPR91 Polyclonal Antibody, Unconjugated secondary primary antibody 1:600, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

Sample: HepG2 Cell (Human) Lysate at 40 ug
Primary: Anti-GPR91(abs123380)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 39kD
Observed band size: 39kD

Protein: 1.kidney lyates(mo);2.liver lyates(mo);
Primary: Rabbit Anti-GPR91 (abs123380) at 1:300;
Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG at 1: 10000;
Predicted band size:39 kDObserved band size:49 kD

Western blot analysis of extracts from Liver tissue(mo) using GPR91 Antibody.

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货号：

abs123380-100ul

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询价

100ul

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