SUMO-1 (h)(rec.)(His)

Produced in E. coli. Human SUMO-1 is fused to a His-tag.

~12kDa

≥95% (SDS-PAGE)

Liquid. In HEPES, pH 8.0, containing 50mM NaCl, 1mM DTT.

小分子泛素样修饰蛋白

In the presence of HeLa Fraction II or a combination of SUMO E1 and E2 (Prod. No. BML-UW9330 and BML-UW9320 respectively), with ATP, Mg2+, and DTT, mature SUMO-1 may be conjugated to soluble proteins. Typical in vitro concentration for non-rate limiting conjugate formation is 200μM to 1mM, depending upon conditions.

Sentrin-1, Small ubiquitin-related modifier 1

P63165