Human Naive/Memory T Cell Panel

Note: The panel is 50 test size. Extra material for each component for an additional 10 tests is provided for instrument set up purposes. Multicolor immunofluorescent staining followed by flow cytometric analysis and sorting has led to the phenotypic and functional characterization of multiple peripheral T cell subsets. Three major T cell subsets have been well characterized, i.e., naïve, memory and effector T cells. Naïve T cells have a relatively homogenous cell surface phenotype. They express high levels of CD45RA and the CD197 (CCR7 chemokine receptor). Effector T cells coexpress variable to low levels of CD45RA and no CD197 whereas memory T cells coexpress variable to low levels of CD45RA and high levels of CD197. The Human Naive/Memory T Cell Panel contains fluorescent antibodies (each optimized at 5 μl per test) that are specific for the cell surface antigens: CD45RA, CD197, CD4 and CD3. The channel for detecting Phycoerythrin or Phycoerythrin protein-tandem conjugated proteins has been left open for inclusion of other markers. The panel was designed to standardize the multicolor staining and flow cytometric characterization of the three major CD4+ T cell subsets that arise as a consequence of development or clonal expansion and differentiation driven by antigenic stimulation (eg, in response to allergens, infectious disease or vaccination).

Note: The panel is 50 test size. Extra material for each component for an additional 10 tests is provided for instrument set up purposes. Multicolor immunofluorescent staining followed by flow cytometric analysis and sorting has led to the phenotypic and functional characterization of multiple peripheral T cell subsets. Three major T cell subsets have been well characterized, i.e., naïve, memory and effector T cells. Naïve T cells have a relatively homogenous cell surface phenotype. They express high levels of CD45RA and the CD197 (CCR7 chemokine receptor). Effector T cells coexpress variable to low levels of CD45RA and no CD197 whereas memory T cells coexpress variable to low levels of CD45RA and high levels of CD197. The Human Naive/Memory T Cell Panel contains fluorescent antibodies (each optimized at 5 μl per test) that are specific for the cell surface antigens: CD45RA, CD197, CD4 and CD3. The channel for detecting Phycoerythrin or Phycoerythrin protein-tandem conjugated proteins has been left open for inclusion of other markers. The panel was designed to standardize the multicolor staining and flow cytometric characterization of the three major CD4+ T cell subsets that arise as a consequence of development or clonal expansion and differentiation driven by antigenic stimulation (eg, in response to allergens, infectious disease or vaccination).

Flow cytometry (Routinely Tested)

Human (QC Testing)

研发参考(5) 1. Appay V, van Lier RA, Sallusto F, Roederer M. Phenotype and function of human T lymphocyte subsets: consensus and issues. Cytometry A. 2008; 73(11):975-983. (Biology). 2. Kallies A. Distinct regulation of effector and memory T-cell differentiation. Immunol Cell Biol. 2008; 86(4):325-332. (Biology). 3. Sallusto F, Geginat J, Lanzavecchia A. Central memory and effector memory T cell subsets: function, generation, and maintenance. Annu Rev Immunol. 2004; 22:745-763. (Biology). 4. Saule P, Trauet J, Dutriez V, Lekeux V, Dessaint JP, Labalette M. Accumulation of memory T cells from childhood to old age: central and effector memory cells in CD4(+) versus effector memory and terminally differentiated memory cells in CD8(+) compartment. 2006; 127(3):274-281. (Biology). 5. Seder RA, Darrah PA, Roederer M. T-cell quality in memory and protection: implications for vaccine design. Nat Rev Immunol. 2008; 8(4):247-258. (Biology).