Mouse Naive/Memory T Cell Panel

Note: The panel is 50 test size. Extra material for each component for an additional 10 tests is provided for instrument set up purposes. The antibodies are supplied in an aqueous buffered solution containing BSA, protein stabilizer, and ≤ 0.09% sodium azide. Multicolor immunofluorescent staining followed by flow cytometric analysis and sorting has led to the phenotypic and functional characterization of multiple peripheral T cell subsets. Three major T cell subsets have been well characterized, i.e., naïve, memory and effector T cells. Naïve mouse T cells have a relatively homogenous cell surface phenotype. They express high levels of the peripheral lymph node homing receptor, CD62L (L-selectin), and low to intermediate levels of the adhesion molecule, CD44 (Pgp-1). Effector T cells coexpress variable to low levels of CD62L and high CD44 levels whereas memory T cells coexpress variable to high levels of CD62L and high levels of CD44. The Mouse Naive/Memory T Cell Panel contains fluorescent antibodies (each optimized at 5 μl per test) that are specific for the cell surface antigens: CD44, CD62L, CD4 and CD3. The panel was designed to standardize the multicolor staining and flow cytometric characterization of the three major CD4+ T cell subsets that arise as a consequence of development or clonal expansion and differentiation driven by antigenic stimulation (eg, in response to allergens, infectious disease or vaccination). This fluorescent antibody panel was flexibly designed to permit the researcher's incorporation of FITC- or Alexa Fluor® 488-conjugated antibodies/probes or cells expressing green fluorescent proteins into flow cytometric analysis of mouse T cell subsets.

Note: The panel is 50 test size. Extra material for each component for an additional 10 tests is provided for instrument set up purposes. The antibodies are supplied in an aqueous buffered solution containing BSA, protein stabilizer, and ≤ 0.09% sodium azide. Multicolor immunofluorescent staining followed by flow cytometric analysis and sorting has led to the phenotypic and functional characterization of multiple peripheral T cell subsets. Three major T cell subsets have been well characterized, i.e., naïve, memory and effector T cells. Naïve mouse T cells have a relatively homogenous cell surface phenotype. They express high levels of the peripheral lymph node homing receptor, CD62L (L-selectin), and low to intermediate levels of the adhesion molecule, CD44 (Pgp-1). Effector T cells coexpress variable to low levels of CD62L and high CD44 levels whereas memory T cells coexpress variable to high levels of CD62L and high levels of CD44. The Mouse Naive/Memory T Cell Panel contains fluorescent antibodies (each optimized at 5 μl per test) that are specific for the cell surface antigens: CD44, CD62L, CD4 and CD3. The panel was designed to standardize the multicolor staining and flow cytometric characterization of the three major CD4+ T cell subsets that arise as a consequence of development or clonal expansion and differentiation driven by antigenic stimulation (eg, in response to allergens, infectious disease or vaccination). This fluorescent antibody panel was flexibly designed to permit the researcher"s incorporation of FITC- or Alexa Fluor® 488-conjugated antibodies/probes or cells expressing green fluorescent proteins into flow cytometric analysis of mouse T cell subsets.

Flow cytometry (Routinely Tested)

Mouse (QC Testing)

研发参考(6) 1. Baaten BJ, Li CR, Deiro MF, Lin MM, Linton PJ, Bradley LM. CD44 regulates survival and memory evelopment in Th1 cells. Cell. 2010; 32(1):104-115. (Biology). 2. Bjorkdahl O, Barber KA, Brett SJ, et al. Characterization of CC-chemokine receptor 7 expression on murine T cells in lymphoid tissues. Immunology. 2003; 110(2):170-179. (Biology). 3. Boyman O, Letourneau S, Krieg C, Sprent J. Homeostatic proliferation and survival of naive and memory T cells. Eur J Immunol. 2009; 39(8):2088-2094. (Biology). 4. Budd RC, Cerottini JC, Horvath C, et al. Distinction of virgin and memory T lymphocytes. Stable acquisition of the Pgp-1 glycoprotein concomitant with antigenic stimulation. J Immunol. 1987; 138(10):3120-3129. (Biology). 5. Ernst DN, Weigle WO, Noonan DJ, McQuitty DN, Hobbs MV. The age-associated increase in IFN-γ synthesis by mouse CD8+ T cells correlates with shifts in the frequencies of cell subsets defined by membrane CD44, CD45RB, 3G11, and MEL-14 expression. J Immunol. 1993; 151(2):575-587. (Biology). 6. Lee WT, Vitetta ES. The differential expression of homing and adhesion molecules on virgin and memory T cells in the mouse. Cell Immunol. 1991; 132(1):215-222. (Biology).