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Dog Activated T Lymphocyte Cocktail
Dog Activated T Lymphocyte Cocktail

Dog Activated T Lymphocyte Cocktail

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品牌： BD Pharmingen
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    反应种属：
    Dog (QC Testing)
    Dog (QC Testing)
    产品介绍
    产品信息
    简单描述
    The Dog Lymphocyte Activation Cocktail is a three-color reagent cocktail designed to identify dog activated lymphocytes by direct immunofluorescent staining with flow cytometric analysis.  The LSM 8.358 antibody reacts with a CD3-like T-cell receptor-associated cell-surface antigen found on thymocytes and peripheral T lymphocytes.  The LSM 11.425 antibody was generated against cells derived from canine peripheral lymph nodes and has been used as a prognostic tool in dog B cell lymphoma studies. The CTL 2.58 antibody was generated by using whole cell immunizations of IL-2 dependent feline T cell lines stimulated with PHA and Con A.  This clone reacts with dog and is referred to as the T Cell Activation Marker.
    商品描述
    The Dog Lymphocyte Activation Cocktail is a three-color reagent cocktail designed to identify dog activated lymphocytes by direct immunofluorescent staining with flow cytometric analysis.  The LSM 8.358 antibody reacts with a CD3-like T-cell receptor-associated cell-surface antigen found on thymocytes and peripheral T lymphocytes.  The LSM 11.425 antibody was generated against cells derived from canine peripheral lymph nodes and has been used as a prognostic tool in dog B cell lymphoma studies. The CTL 2.58 antibody was generated by using whole cell immunizations of IL-2 dependent feline T cell lines stimulated with PHA and Con A.  This clone reacts with dog and is referred to as the T Cell Activation Marker.
    BD化合物表
    • 描述
      数量/尺寸
      零件号
      EntrezGene ID
    • APC Mouse anti-Dog Pan T Cell
      N/A
      51-590-9005060
      N/A
    • Multiple Mouse anti-Dog B Cells
      N/A
      51-550-9003756
      N/A
    • FITC Mouse anti-Dog Activated T cells
      N/A
      51-540-9005068
      N/A
    应用
    反应种属
    Dog (QC Testing)
    文献
    文献
    研发参考(3) 1. Cobbold S, Metcalfe S. Monoclonal antibodies that define canine homologues of human CD antigens: Summary of the First International Canine Leukocyte Antigen Workshop (CLAW). Tissue Antigens. 1994; 43:137-154. (Clone-specific). 2. Gebhard GH, Carter PB. Identification of canine T-lymphocyte subsets with monoclonal antibodies. Vet Immunol Immunopathol. 1992; 33:187-199. (Biology). 3. Ruslander DA, Gebhard DH, Tompkins MB, Grindem CB, Page RL. Immunophenotypic characterization of canine lymphoproliferative disorders. In Vivo. 1997; 11(2):169-172. (Biology).

    参考图片

    Three-color analysis of the expression of LSM 8.358 (CD3/Pan T marker), LSM 11.425 (B cell marker) and CTL 2.58 (activation marker) on lysed whole blood from Canine . PBMC from Dog was stained with either Isotype Control Cocktail A (Cat. no. 558509; data not shown) or Dog Activated T Lymphocyte Cocktail (Cat. no. 558704). During data analysis, lymphocytes were identified by scatter profile. The figure on the left represents the LSM 8.358 (Pan T marker) and CTL 2.58 (activation marker) profile on resting lymphocytes while the figure in the middle represents these markers on Concanavalin A stimulated PBMC. The panel on the right represents the profile for LSM 8.58 (Pan T marker) and LSM 11.425 (B cell marker) also on Concanavalin A stimulated cells. Flow cytometry was performed on a BD FACSCalibur™.

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    货号：
    558704
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