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品牌： R&D Systems
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Product Summary
The Parameter TBARS assay is a 2-3 hour chemical analysis designed to measure TBARS in cell culture supernates, cell lysates, serum, plasma, and urine.
Recovery
The recovery of MDA spiked to levels throughout the range of the assay in various matrices was evaluated.
| Sample Type | Average % Recovery | Range % |
|---|---|---|
| Cell Culture Media (n=4) | 101 | 94-110 |
| Cell Lysis Buffer 3 (n=1) | 99 | 96-103 |
| EDTA Plasma (n=4) | 91 | 89-106 |
| Heparin Plasma (n=4) | 99 | 94-108 |
| Serum (n=4) | 98 | 87-105 |
| Urine (n=4) | 99 | 91-106 |
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of MDA, acid treated, and serially diluted with deionized water to produce samples with values within the dynamic range of the assay.
Scientific Data
Assay Procedure
Refer to the product Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
- Prepare all reagents, TBARS standard, and samples as directed in the product insert.
- Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
- Add 150 µL of standards and samples to each well.
- Add 75 µL of TBA Reagent to each well.
- Pre-read the optical density of each well using a microplate reader set to 532 nm.
- Incubate the microplate for 2-3 hours at 45-50 °C.
- Read the optical density of each well using a microplate reader set to 532 nm. Subtract the pre-reading from the final reading to correct for the sample's contributions to the final absorption at 532 nm.
150 µL Standards and Samples
75 µL TBA Reagent
TBARS Parameter Assay Kit Summary
Format
96-well strip plate
Assay Length
2-3 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (150 uL), Cell Lysates (150 uL), Serum (150 uL), EDTA Plasma (150 uL), Heparin Plasma (150 uL), Urine (150 uL)
Sensitivity
0.055 uM
Assay Range
0.0 - 17 uM
Specificity
TBARS in various samples
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.
背景
研究领域
制备和贮存
保存方式
Preparation and Storage
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
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危险品化学品经营许可证(不带存储) 许可证编号：沪(杨)应急管危经许[2022]202944(QY) 
营业执照(三证合一)