
产品介绍
产品信息
简单描述
The BD™ CBA Human Th1/Th2 Cytokine Kit (Catalog No. 550749) can be used to quantitatively measure Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-5 (IL-5), Interleukin-10 (IL-10), Tumor Necrosis Factor (TNF), and Interferon-γ (IFN-γ) protein levels in a single sample. The kit performance has been optimized for analysis of specific cytokines in tissue culture supernatants, EDTA plasma, and serum samples. The kit provides sufficient reagents for the quantitative analysis of 80 samples.

商品描述
The BD™ CBA Human Th1/Th2 Cytokine Kit (Catalog No. 550749) can be used to quantitatively measure Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-5 (IL-5), Interleukin-10 (IL-10), Tumor Necrosis Factor (TNF), and Interferon-γ (IFN-γ) protein levels in a single sample. The kit performance has been optimized for analysis of specific cytokines in tissue culture supernatants, EDTA plasma, and serum samples. The kit provides sufficient reagents for the quantitative analysis of 80 samples.

BD化合物表
- 描述数量/尺寸零件号EntrezGene ID
- C) Human Th1/Th2 Cytokine StandardsN/A51-2428KCN/A
- E1) PE Positive Control DetectorN/A51-2429KCN/A
- B Human Th1/Th2 PE Detection ReagentN/A51-2427KCN/A
- Human IFN-γ Capture Beads1 Each (1 ea)51-9005727N/A
- Human TNF Capture Beads1 Each (1 ea)51-9005724N/A
- Human IL-10 Capture Beads80 Tests (1 ea)51-9005740N/A
- Human IL-5 Capture Beads1 Each (1 ea)51-9005718N/A
- Human IL-4 Capture Beads1 Each (1 ea)51-9005715N/A
- Human IL-2 Capture Beads80 Tests (1 ea)51-9005736N/A
- D Cytometer Setup Beads1 Each (1 ea)51-9005731N/A
- BD(tm) CBA Serum Enhancement Buffer (H)N/A51-9002150N/A
- G Assay Diluent1 Each (1 ea)51-2432KCN/A
- F Wash Buffer1 Each (1 ea)51-2431KCN/A
- E2 FITC Positive Control Detector1 Each (1 ea)51-9005734N/A
- E1 PE Positive Control Detector1 Each (1 ea)51-9005732N/A
应用
反应种属
Human

文献
文献
研发参考(13)
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2. Camilla C, Defoort JP, Delaage M, et al. A new flow cytometry-based multi-assay system 1, Application to cytokine immunoassays. Cytometry Suppl. 1998; 8:132. (Biology).
3. Carson R, Vignali D. Simultaneous quantitation of 15 cytokines using a multiplexed flow cytometric assay. J Immunol Methods. 1999; 227:41–52. (Biology).
4. Chen, R, L Lowe, et al. Simultaneous quantification of six human cytokines in a single sample using microparticle-based flow cytometric technology. Clin Chem. 1999; 9:1693-1694. (Biology).
5. Collins DP, Luebering BJ, Shaut DM. T-lymphocyte functionality assessed by analysis of cytokine receptor expression, intracellular cytokine expression, and femtomolar detection of cytokine secretion by quantitative flow cytometry. Cytometry. 1998; 33:249-255. (Biology).
6. Cook, EB, JL Stahl, et al. Simultaneous measurement of six cytokines in a single sample of human tears using microparticle-based flow cytometry: allergics vs non-allergics. J Immunol Methods. 2001; 254:109-118. (Biology).
7. Dotti, G, B Salvodo, et al. Adenovector-induced expression of human-CD40-ligand (hCD40L) by multiple myeloma cells: A model for immunotherapy. Exp Hematol. 2001; 29:952-961. (Biology).
8. Fulton RJ, McDade RL, Smith PL, Kienker LJ, Kettman JR Jr. Advanced multiplexed analysis with the FlowMetrix system. Clin Chem. 1997; 43:1749-1756. (Biology).
9. Kricka LJ. Diamandis EP, Christopoulos TK, ed. Immunoassay. Academic Press; 1996:389-404.
10. Lund-Johansen F, Davis K, Bishop J, de Waal Malefyt R. Flow cytometric analysis of immunoprecipitates: high-throughput analysis of protein phosphorylation and protein-protein interactions. Cytometry. 2000; 39(4):250-259. (Biology).
11. McHugh TM. Flow microsphere immunoassay for the quantitative and simultaneous detection of multiple soluble analytes. Methods Cell Biol. 1994; 33:613-629. (Biology).
12. Oliver KG, Kettman JR, Fulton RJ. Multiplexed analysis of human cytokines by use of the FlowMetrix system. Clin Chem. 1998; 44:2057-2060. (Biology).
13. Stall, A, Q Sun, et al. A single tube flow cytometric multibead assay for isotyping mouse monoclonal antibodies, Abstract LB77. Experimental Biology Meeting. 1998. (Biology).

参考图片
Resolution of the individual bead populations in the BD CBA Human Th1/Th2 Cytokine Kit. Each population corresponds to an individual analyte.
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