首页
THUNDER™ cAMP TR-FRET Assay Kit
品牌： Bioauxilium
下载产品说明书
用小程序，查商品更便捷
收藏
对比
咨询反应种属：
All species expected
All species expected
来源宿主：
鼠
鼠
产品介绍
产品信息
抗原名称
cAMP
宿主
鼠
商品描述
Homogeneous competitive TR-FRET immunoassay for quantitative measurement of cAMP in cell lysates. This is a robust, sensitive, and easy-to-use assay for monitoring GPCR activation based on cAMP production in cells. The kit is compatible with both suspension and adherent cells and is suitable for the profiling and high-throughput screening of Gαs- and Gαi-coupled GPCRs. Detailed assay protocols for testing agonists and antagonists, as well as full assay validation can be found in the Technical Data Sheet. BEST SELLER
组成成分
8.3%腺苷3',5'-环单磷酸，2.08%生物素化腺苷3',5'-环单磷酸，2.08%链霉亲和素，4.2%抗腺苷3',5'-环单磷酸抗体，83.34%检测缓冲液（磷酸盐缓冲液）
Components
cAMP standard (100 µL)
Biotin-cAMP (500 µL)
Eu-streptavidin (500 µL)
Acc-anti-cAMP (1 mL)
5X cAMP Detection Buffer (40 mL)
Reactivity
All species expected
Assay principle: Free cAMP produced by cells competes with the Europium labeled streptavidin/biotin-cAMP tracer complex for binding to a monoclonal cAMP antibody labeled with the far-red fluorophore. The specific TR-FRET signal is therefore inversely proportional to the concentration of cAMP in the lysate.
The THUNDER™ cAMP assay exhibits a comparable performance to that of a competing TR-FRET assay.
The THUNDER™ cAMP assay workflow consists of only two steps.
应用
反应种属
All species expected
声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。
危险品化学品经营许可证(不带存储) 许可证编号：沪(杨)应急管危经许[2022]202944(QY) 
营业执照(三证合一)