THUNDER™ High Performance Phospho-STAT6 (Y641) TR-FRET Cell Signaling Assay Kit

品牌： Bioauxilium

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反应种属：

Human, Mouse

产品介绍

产品信息

抗原名称

STAT6

商品描述

High performance sandwich TR-FRET immunoassay for semi-quantitative detection of Human and Mouse Phospho-STAT6 (Y641) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Note: This kit is a new and improved high performance version of catalog number KIT-STAT6P-XXX, which will remain available for purchase.

All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Components
Eu-HP-phospho-STAT6 (20 µL)
Acc-HP-phospho-STAT6 (80 µL)
STAT6 control lysate (200 µL)
5X Lysis Buffer 8 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)

Reactivity
Human, Mouse

High Performance Phospho-STAT6 vs Current Kit

Data show the superior S/B ratio obtained with the High Performance Phospho-STAT6 (Y641) Kit compared to the standard version of the kit.

Stimulation of Phospho-STAT6 (Y641) in HeLa cells

Data show that treatment of HeLa cells with IL-4 stimulates phosphorylation of STAT6 at Y641. Robust S/B ratio and pharmacology can be obtained after 1h of incubation.

Inhibition of Phospho-STAT6 (Y641) in HeLa cells

Data show that treatment of HeLa cells with JAK Inhibitor 1 inhibits phosphorylation of STAT6 at Y641 by IL-4. Robust S/B ratio and pharmacology can be obtained after 1h of incubation.