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THUNDER™ Phospho-4EBP1 (T37/T46) TR-FRET Cell Signaling Assay Kit
THUNDER™ Phospho-4EBP1 (T37/T46) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-4EBP1 (T37/T46) TR-FRET Cell Signaling Assay Kit

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品牌： Bioauxilium
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    反应种属：
    Human
    Human
    产品介绍
    产品信息
    抗原名称
    4EBP1
    商品描述

    Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-4EBP1 (T37/T46) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. Assay benchmarking is shown in the updated Application Note.

    Components
    Eu-phospho-4EBP1 (25 µL)
    Acc-phospho-4EBP1 (100 µL)
    4EBP1 control lysate (200 µL)
    5X Lysis Buffer 2 (5 mL)
    10X TR-FRET Detection buffer (250 µL)
    100X Phosphatase Inhibitor Cocktail (250 µL)

    Reactivity
    Human

    Inhibition of Phospho 4EBP1 (T37-T46)in A431 cells
    Treatment of A431 cells with PP242 inhibits phosphorylation of 4EBP1 at T37/T46.
    Inhibition of Phospho 4EBP1 (T37-T46) in HEK293 cells
    Treatment of HEK293 cells with PP242 inhibits phosphorylation of 4EBP1 at T37/T46
    Z-factor determination in A431 cells
    Z’-factor value of 0.73 indicates that the assay is robust and suitable for HTS
    A431 control lysate titration (QC test)
    Data show a linear relationship between lysate dilutions and TR-FRET ratio values
    应用
    反应种属
    Human
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    货号：
    KIT-4EBP1P-500
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    询价
    500points
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