THUNDER™ Phospho-AKT pan (S473) TR-FRET Cell Signaling Assay Kit

品牌： Bioauxilium

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反应种属：

Human, Mouse

来源宿主：

鼠

产品介绍

产品信息

抗原名称

AKT pan

宿主

鼠

商品描述

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-AKT1/2/3 (S473) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. Assay benchmarking is shown in the updated Application Note. The THUNDER™ assay outperforms the two other TR-FRET assays available. BEST SELLER

组成成分

磷酸化蛋白激酶B抗体(铕离子标记)，磷酸化蛋白激酶B抗体(远红光受体标记)，裂解缓冲液（聚乙二醇单辛基苯基水溶液），检测缓冲液（磷酸盐缓冲液），细胞裂解液（三(羟甲基)氨基甲烷溶液），磷酸酶抑制剂混合物（N-(2，2，2-三氯-1-(3-(喹啉-8-基)硫脲基)乙基)肉桂酰胺，水）

Components
Eu-phospho-AKTS473 (25 µL)
Acc-phospho-AKTS473 (100 µL)
AKT control lysate (200 µL)
5X Lysis Buffer 7 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)

Reactivity
Human, Mouse

Stimulation of Phospho-AKT pan (S473) NIH3T3cells

Treatment of NIH3T3 cells with PDGF-AA stimulates phosphorylation of AKT pan at S473.

Inhibition Phospho-AKT pan (S473) in NIH3T3 cells

Treatment of NIH3T3 cells with Wortmannin inhibits phosphorylation of AKT pan at S473.

Z'-factor determinationPhospho in NIH3T3 cells

The Z’-factor value of 0.74 indicates that the assay is robust and suitable for HTS.

Data show a linear relationship between lysate dilutions and TR FRET ratio values.

Inhibition Phospho-AKT pan (S473) in Jurkat cells

Treatment of Jurkat cells with LY294002 inhibits phosphorylation of AKT pan at S473.

Data show that the THUNDER™ assay kit outperforms the other two competing TR-FRET kits.

应用

反应种属

Human, Mouse

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货号：

KIT-AKTS473P-500

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