THUNDER™ Phospho-AKT pan (S473) + Total AKT pan TR-FRET Cell Signaling Assay Kit

品牌： Bioauxilium

下载产品说明书

用小程序，查商品更便捷

对比

咨询

参数

反应种属：

Human, Mouse

产品介绍

产品信息

抗原名称

AKT pan

商品描述

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-AKT 1/2/3 (S473) and Total AKT 1/2/3 in cell lysates. This unique kit allows to simultaneously detect phosphorylated and total AKT pan proteins from the same lysate sample in separate wells within the same microplate. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

400 points for phospho; 100 points for total

Components
Eu-phospho-AKTS473 (20 µL)
Acc-phospho-AKTS473 (80 µL)
Eu-Total AKT (5 µL)
Acc-Total AKT (20 µL)
AKT control lysate (200 µL)
5X Lysis Buffer 7 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)

Reactivity
Human, Mouse

Stimulation of Phospho-Total AKT pan (S473) in NIH3T3 cells

Data show that treatment of NIH3T3 cells with PDGF-AA stimulates phosphorylation of AKT pan at S473 but does not affect the levels of total AKT pan.

Inhibition of Phospho-Total AKT pan (S473) in NIH3T3 cells

Data show that treatment of NIH3T3 cells with Wortmannin inhibits phosphorylation of AKT pan at S473 but does not affect the levels of total AKT pan.

NIH3T3 contro lysate titration (QC test) Phospho-AKT pan (S473)

Data show a linear relationship between lysate dilutions and TR-FRET ratio values.

NIH3T3 control lysate titration (QC test) Total AKT pan

Data show a linear relationship between lysate dilutions and TR-FRET ratio values.

应用

反应种属

Human, Mouse

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

KIT-AKTS473PT-500

一键复制

询价

500points

选择数量

当前规格1件起购

配送至

预计2-3周送达，快递: 免运费，若需干冰额外收费

询价大包装询价