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THUNDER™ Phospho-AKT pan (T308) TR-FRET Cell Signaling Assay Kit
THUNDER™ Phospho-AKT pan (T308) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-AKT pan (T308) TR-FRET Cell Signaling Assay Kit

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品牌： Bioauxilium
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对比对比
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    反应种属：
    Human, Mouse
    Human, Mouse
    来源宿主：
    鼠、大肠杆菌
    鼠、大肠杆菌
    产品介绍
    产品信息
    抗原名称
    AKT pan
    宿主
    鼠、大肠杆菌
    商品描述

    Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-AKT 1/2/3 (T308) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

    组成成分
    磷酸化蛋白激酶B抗体(铕离子标记)0.4%，磷酸化蛋白激酶B抗体(远红光受体标记)1.6%，裂解缓冲液 （聚乙二醇单辛基苯基水溶液）81.8%，检测缓冲液（磷酸盐缓冲液）4%，细胞裂解液（重组蛋白激酶B蛋白，三(羟甲基)氨基甲烷）8.2%，磷酸酶抑制剂混合物（N-(2，2，2-三氯-1-(3-(喹啉-8-基)硫脲基)乙基)肉桂酰胺，水）4%

    Components
    Eu-phospho-AKTT308 (25 µL)
    Acc-phospho-AKTT308 (100 µL)
    AKT control lysate (200 µL)
    5X Lysis Buffer 1 (5 mL)
    10X TR-FRET Detection buffer (250 µL)
    100X Phosphatase Inhibitor Cocktail (250 µL)

    Reactivity
    Human, Mouse

    Stimulation of Phospho-AKT pan (T308) in NIH3T3 cells
    Data show that treatment of NIH3T3 cells with PDGF-AA stimulates phosphorylation of AKT at T308.
    Inhibition of Phospho-AKTpan(T308) in NIH3T3 cells
    Data show that treatment of NIH3T3 cells with Wortmannin inhibits phosphorylation of AKT at T308 by PDGF-AA.
    Z'-factor determination-NIH3T3-cells
    The Z’-factor value of 0.7 indicates that the assay is robust and suitable for HTS.
    应用
    反应种属
    Human, Mouse
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    货号：
    KIT-AKTT308P-500
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    询价
    500points
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