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THUNDER™ Phospho-BAD (S112) TR-FRET Cell Signaling Assay Kit

产品介绍
产品信息
抗原名称
BAD

商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-BAD (S112) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
Components
Eu-phospho-BAD (25 µL)
Acc-phospho-BAD (100 µL)
BAD control lysate (500 µL)
5X Lysis Buffer 1 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)
Reactivity
Human

Data show that treatment of MCF7 cells with PMA stimulates phosphorylation of BAD at S112

Data show that treatment of MCF7 cells with UCN-01 inhibits phosphorylation of BAD at S112 by PMA

The Z’-factor value of 0.76 indicates that the assay is robust and suitable for HTS

Data show a linear relationship between lysate dilutions and TR-FRET ratio values
应用
反应种属
Human

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