THUNDER™ Phospho-BAD (S112) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-BAD (S112) TR-FRET Cell Signaling Assay Kit

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品牌: Bioauxilium
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    反应种属:
    Human
    Human
    产品介绍
    产品信息
    抗原名称
    BAD
    商品描述

    Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-BAD (S112) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

    Components
    Eu-phospho-BAD (25 µL)
    Acc-phospho-BAD (100 µL)
    BAD control lysate (500 µL)
    5X Lysis Buffer 1 (5 mL)
    10X TR-FRET Detection buffer (250 µL)
    100X Phosphatase Inhibitor Cocktail (250 µL)

    Reactivity
    Human

    Stimulation of Phospho-BAD (S112) in MCF7 cells
    Data show that treatment of MCF7 cells with PMA stimulates phosphorylation of BAD at S112
    Inhibition of Phospho-BAD (S112) in MCF7 cells
    Data show that treatment of MCF7 cells with UCN-01 inhibits phosphorylation of BAD at S112 by PMA
    Z'-factor determination in MCF7 cells
    The Z’-factor value of 0.76 indicates that the assay is robust and suitable for HTS
    HEK293 control lysate titration (QC Test)
    Data show a linear relationship between lysate dilutions and TR-FRET ratio values
    应用
    反应种属
    Human
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    货号:
    KIT-BADP-500
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