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THUNDER™ Phospho-NF-kB (S536) TR-FRET Cell Signaling Assay Kit

产品介绍
产品信息
抗原名称
NF-κB

宿主
鼠、大肠杆菌

商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-NF-κB (S536) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.
Product citation: PubMed 36129967
组成成分
磷酸化核因子蛋白抗体(铕离子标记)0.4%,磷酸化核因子蛋白抗体(远红光受体标记)1.6%,裂解缓冲液(聚乙二醇单辛基苯基水溶液)81.6%,检测缓冲液(磷酸盐缓冲液)4.1%,细胞裂解液(磷酸化核因子重组蛋白,三(羟甲基)氨基甲烷)8.2%,磷酸酶抑制剂混合物(N-(2,2,2-三氯-1-(3-(喹啉-8-基)硫脲基)乙基)肉桂酰胺,水)4.1%

Components
Eu-phospho-NFkB (25 µL)
Acc-phospho-NFkB (100 µL)
NFkB control lysate (200 µL)
5X Lysis Buffer 2 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (50 µL)
Reactivity
Human

Data show that treatment of HeLa cells with TNFα stimulates phosphorylation of NF-kB at S536

Data show that treatment of HeLa cells with IKK-16 inhibits phosphorylation of NF-kB at S536 by TNFα

The Z’-factor value of 0.78 indicates that the assay is robust and suitable for HTS

Data show a linear relationship between lysate dilutions and TR-FRET ratio values
应用
反应种属
Human

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