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THUNDER™ Phospho-p38α MAPK (T180/Y182) TR-FRET Cell Signaling Assay Kit
THUNDER™ Phospho-p38α MAPK (T180/Y182) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-p38α MAPK (T180/Y182) TR-FRET Cell Signaling Assay Kit

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品牌： Bioauxilium
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    反应种属：
    Human
    Human
    来源宿主：
    鼠
    鼠
    产品介绍
    产品信息
    抗原名称
    p38α MAPK
    宿主
    鼠
    商品描述

    Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p38α MAPK14 (T180/Y182) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

    组成成分
    磷酸化P38α丝裂原活化蛋白激酶抗体(铕离子标记）0.4%，磷酸化P38α丝裂原活化蛋白激酶抗体(远红光受体标记)1.6%，裂解缓冲液（聚乙二醇单辛基苯基水溶液）81.6%，检测缓冲液（磷酸盐缓冲液）4.1%，细胞裂解液（三(羟甲基)氨基甲烷）8.2%，磷酸酶抑制剂混合物 （N-(2，2，2-三氯-1-(3-(喹啉-8-基)硫脲基)乙基)肉桂酰胺，水）4.1%

    Components
    Eu-phospho-p38a (25 µL)
    Acc-phospho-p38a (100 µL)
    p38a control lysate (200 µL)
    5X Lysis Buffer 6 (5 mL)
    10X TR-FRET Detection buffer (250 µL)
    100X Phosphatase Inhibitor Cocktail (250 µL)

    Reactivity
    Human

    Stimulation of Phospho-p38α (T180-Y182) in HeLa cells
    Data show that treatment of HeLa cells with Anisomycin stimulates phosphorylation of p38α at T180/Y182
    Z'-factor determination in HeLa cells
    The Z’-factor value of 0.71 indicates that the assay is robust and suitable for HTS.
    HeLa Control lysate titration (QC Test)
    Data show a linear relationship between lysate dilutions and TR-FRET ratio values.
    应用
    反应种属
    Human
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    货号：
    KIT-P38AP-500
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    询价
    500points
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