THUNDER™ Phospho-p38αβγ MAPK (T180/Y182) TR-FRET Cell Signaling Assay Kit

品牌： Bioauxilium

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参数

反应种属：

Human

来源宿主：

鼠

产品介绍

产品信息

抗原名称

p38αβγ MAPK

宿主

鼠

商品描述

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p38αβγ MAPK14/11/12 (T180/Y182) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. Assay benchmarking is shown in the updated Application Note. The THUNDER™ assay outperforms the two other TR-FRET assays available.

组成成分

磷酸化P38αβγ丝裂原活化蛋白激酶抗体(铕离子标记）0.4%，磷酸化P38αβγ丝裂原活化蛋白激酶抗体(远红光受体标记)1.6%，裂解缓冲液（聚乙二醇单辛基苯基水溶液）81.6%，检测缓冲液（磷酸盐缓冲液）4.1%，细胞裂解液（三(羟甲基)氨基甲烷）8.2%，磷酸酶抑制剂混合物（N-(2，2，2-三氯-1-(3-(喹啉-8-基)硫脲基)乙基)肉桂酰胺，水）4.1%

Components
Eu-phospho-p38 (25 µL)
Acc-phospho-p38 (100 µL)
p38a control lysate (200 µL)
5X Lysis Buffer 6 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)

Reactivity
Human