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THUNDER™ Phospho-p53 (S15) TR-FRET Cell Signaling Assay Kit
THUNDER™ Phospho-p53 (S15) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-p53 (S15) TR-FRET Cell Signaling Assay Kit

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品牌： Bioauxilium
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    反应种属：
    Human
    Human
    产品介绍
    产品信息
    抗原名称
    p53
    商品描述

    Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-p53 (S15) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

    Components
    Eu-phospho-p53 (25 µL)
    Acc-phospho-p53 (100 µL)
    p53 control lysate (200 µL)
    5X Lysis Buffer 2 (5 mL)
    10X TR-FRET Detection buffer (250 µL)
    100X Phosphatase Inhibitor Cocktail (250 µL)

    Reactivity
    Human

    Stimulation of Phospho-P53 (S15) in HEK293 cells
    Data show that treatment of HEK293 cells with Etoposide stimulates phosphorylation of p53 at Ser15
    Z'-factor determination in HEK293 cells
    The Z’-factor value of 0.6 indicates that the assay is robust and suitable for HTS
    HEK293 control lysate titration (QC Test)
    Data show a linear relationship between lysate dilutions and TRFRET ratio values
    应用
    反应种属
    Human
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    货号：
    KIT-P53P-500
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