THUNDER™ Phospho-STAT5 (Y694/Y699) TR-FRET Cell Signaling Assay Kit

THUNDER™ Phospho-STAT5 (Y694/Y699) TR-FRET Cell Signaling Assay Kit

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品牌: Bioauxilium
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    反应种属:
    Human, Mouse
    Human, Mouse
    来源宿主:
    产品介绍
    产品信息
    抗原名称
    STAT5
    宿主
    商品描述

    Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT5 (Y694) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. BEST SELLER

    Product citation: PubMed 37698877; PubMed 34570089

    Components
    Eu-phospho-STAT5 (25 µL)
    Acc-phospho-STAT5 (100 µL)
    STAT5 control lysate (200 µL)
    5X Lysis Buffer 2 (5 mL)
    10X TR-FRET Detection buffer (250 µL)
    100X Phosphatase Inhibitor Cocktail (250 µL)

    Reactivity
    Human, Mouse

    Stimulation of Phospho-STAT5 (Y694/Y699) in  A431 cells
    Data show that treatment of A431 cells with EGF stimulates phosphorylation of STAT5 at Y694/Y699.
    Inhibition of Phospho-STAT5 (Y694/Y699) in  A431 cells
    Data show that treatment of A431 cells with Erlotinib inhibits phosphorylation of STAT5 at Y694/Y699 by EGF
    Z'-factor determination in A431 cells
    The Z’-factor value of 0.78 indicates that the assay is robust and suitable for HTS
    A431 control lysate titration (QC Test)
    Data show a linear relationship between lysate dilutions and TR FRET ratio values
    应用
    反应种属
    Human, Mouse
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    货号:
    KIT-STAT5P-500
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