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THUNDER™ Phospho-STAT5 (Y694/Y699) TR-FRET Cell Signaling Assay Kit

产品介绍
产品信息
抗原名称
STAT5

宿主
鼠

商品描述
Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-STAT5 (Y694) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual. BEST SELLER
Product citation: PubMed 37698877; PubMed 34570089
Components
Eu-phospho-STAT5 (25 µL)
Acc-phospho-STAT5 (100 µL)
STAT5 control lysate (200 µL)
5X Lysis Buffer 2 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)
Reactivity
Human, Mouse

Data show that treatment of A431 cells with EGF stimulates phosphorylation of STAT5 at Y694/Y699.

Data show that treatment of A431 cells with Erlotinib inhibits phosphorylation of STAT5 at Y694/Y699 by EGF

The Z’-factor value of 0.78 indicates that the assay is robust and suitable for HTS

Data show a linear relationship between lysate dilutions and TR FRET ratio values
应用
反应种属
Human, Mouse

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