THUNDER™ Phospho-TBK1 (S172) TR-FRET Cell Signaling Assay Kit

品牌： Bioauxilium

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参数

反应种属：

Human

产品介绍

产品信息

抗原名称

TBK1

商品描述

Sandwich TR-FRET immunoassay for semi-quantitative detection of Phospho-TBK1/NAK (S172) in cell lysates. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

Components
Eu-phospho-TBK1 (250 µL)
Acc-phospho-TBK1 (1 mL)
TBK1 control lysate (2 x 200 µL)
5X Lysis Buffer 2 (50 mL)
10X TR-FRET Detection buffer (2.5 mL)
100X Phosphatase Inhibitor Cocktail (2.5 mL)

Reactivity
Human

Data show that treatment of MCF7 cells with Calyculin A stimulates phosphorylation of TBK1/NAK at S172.

Data show that treatment of MCF7 cells with BX-795 inhibits phosphorylation of TBK1/NAK at S172 by Calyculin A.

The Z’-factor value of 0.70 indicates that the assay is robust and suitable for HTS.

Data show a linear relationship between lysate dilutions and TR-FRET ratio values.

Data show the robustness of the assay following overnight incubation.

应用

反应种属

Human

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货号：

KIT-TBK1P-5000

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5000points

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