THUNDER™ Total AKT pan TR-FRET Cell Signaling Assay Kit

品牌： Bioauxilium

下载产品说明书

用小程序，查商品更便捷

对比

咨询

参数

反应种属：

Human

来源宿主：

鼠、大肠杆菌

产品介绍

产品信息

抗原名称

AKT pan

宿主

鼠、大肠杆菌

商品描述

Sandwich TR-FRET immunoassay for semi-quantitative detection of Total AKT 1/2/3 (both phosphorylated and non-phosphorylated AKT1/2/3) in cell lysates. This kit can be used to normalize data obtained with the Phospho-AKT pan (S473) kit and the Phospho-AKT pan (T308) kit. Full assay validation can be found in the Technical Data Sheet. Detailed assay protocols are described in the Generic User Manual.

组成成分

总蛋白激酶B抗体(铕离子标记)0.4%，总蛋白激酶B抗体(远红光受体标记)1.6%，裂解缓冲液（聚乙二醇单辛基苯基水溶液）81.6%，检测缓冲液（磷酸盐缓冲液）4.1%，细胞裂解液（总蛋白激酶B重组蛋白，三(羟甲基)氨基甲烷）8.2%，磷酸酶抑制剂混合物（N-(2，2，2-三氯-1-(3-(喹啉-8-基)硫脲基)乙基)肉桂酰胺，水）4.1%

Components
Eu-Total AKT (25 µL)
Acc-Total AKT (100 µL)
AKT control lysate (200 µL)
5X Lysis Buffer 7 (5 mL)
10X TR-FRET Detection buffer (250 µL)
100X Phosphatase Inhibitor Cocktail (250 µL)

Reactivity
Human

Stimulation of Phospho-AKT pan (S473) in NIH3T3 cells

Data show that treatment of NIH3T3 cells with PDGF-AA stimulates phosphorylation of AKT pan at S473 but does not affect the levels of total AKT pan.

Inhibition of Phospho-AKT pan (S473) in NIH3T cells

Data show that treatment of NIH3T3 cells with Wortmannin inhibits phosphorylation of AKT pan at S473 by PDGF-AA but does not affect the levels of total AKT pan.

NIH3T3 control lysate titration (QC test)

Data show a linear relationship between lysate dilutions and TR-FRET ratio values.

应用

反应种属

Human

声明：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。

货号：

KIT-AKTT-500

一键复制

询价

优宁维现货

500points

选择数量

当前规格1件起购

配送至

预计3-4个工作日送达，快递: 免运费，若需干冰额外收费

询价大包装询价