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Human TNF Flex Set
Human TNF Flex Set
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Human TNF Flex Set

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品牌： BD Pharmingen
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    反应种属：
    Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
    Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)
    产品介绍
    产品信息
    抗原名称
    TNF
    简单描述
    The BD™ CBA Human TNF Flex Set is a bead-based immunoassay capable of measuring human tumor necrosis factor (TNF), formerly known as tumor necrosis factor-α, in serum, plasma, and cell culture supernatant samples. Human and non-human primate reactivity was determined by testing samples with the BD CBA Human TNF Flex Set. The biology and function of TNF has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264 or 558265).
    商品描述
    The BD™ CBA Human TNF Flex Set is a bead-based immunoassay capable of measuring human tumor necrosis factor (TNF), formerly known as tumor necrosis factor-α, in serum, plasma, and cell culture supernatant samples. Human and non-human primate reactivity was determined by testing samples with the BD CBA Human TNF Flex Set. The biology and function of TNF has been extensively reviewed in the literature. For more information on bead-based immunoassays, refer to the product insert for the BD CBA Human Soluble Protein Master Buffer Kit (Cat. No. 558264 or 558265).
    BD化合物表
    • 描述
      数量/尺寸
      零件号
      EntrezGene ID
    • Human TNF Capture Bead D9
      1 Vial(s) (1 ea)
      51-9005298
      N/A
    • Human TNF PE Detection Reagent
      N/A
      51-9004040
      N/A
    • Human TNF Standard
      1 Vial(s) (2 ea)
      51-9003509
      N/A
    应用
    反应种属
    Human (QC Testing), Rhesus, Cynomolgus, Baboon (Tested in Development)

    参考图片

    Figure 1. Example BD CBA Human TNF Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analyzed using the FCAP Array Software (Cat. No. 641488).

    Reproducibility: The inter-assay and intra-assay reproducibility were determined for the BD CBA Human TNF Flex Set by evaluating ten replicates of three different sample levels (inter-assay) and two replicates of three different sample levels from four separate experiments (intra-assay) respectively.

    Recovery:        Cell culture supernatant, serum, or EDTA-treated plasma were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum and plasma samples were diluted 1:4 before the protein was spiked into each. Serum is a pool of 800 - 1000 donors and the plasma was pooled from at least 20 donors.

    Linearity: Cell culture supernatant, 1:4 diluted serum, or 1:4 diluted EDTA-treated plasma were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.

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    货号：
    558273
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