BD™ Cytometric Bead Array (CBA) Human TNF Flex Set

Reproducibility: The inter-assay and intra-assay reproducibility were determined for the BD CBA Human TNF Flex Set by evaluating ten replicates of three different sample levels (inter-assay) and two replicates of three different sample levels from four separate experiments (intra-assay) respectively.

Recovery:        Cell culture supernatant, serum, or EDTA-treated plasma were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum and plasma samples were diluted 1:4 before the protein was spiked into each. Serum is a pool of 800 - 1000 donors and the plasma was pooled from at least 20 donors.

Linearity: Cell culture supernatant, 1:4 diluted serum, or 1:4 diluted EDTA-treated plasma were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.

Figure 1. Example BD CBA Human TNF Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analzed using the FCAP Array Software (Cat. No. 641488).

Figure 1. Example BD CBA Human TNF Flex Set standard curve. Data acquired on a BD FACSArray bioanalyzer and analzed using the FCAP Array Software (Cat. No. 641488).

Recovery:        Cell culture supernatant, serum, or EDTA-treated plasma were spiked with three different levels of protein. The spiked samples were assayed and the results were compared with expected values. Serum and plasma samples were diluted 1:4 before the protein was spiked into each. Serum is a pool of 800 - 1000 donors and the plasma was pooled from at least 20 donors.

Linearity: Cell culture supernatant, 1:4 diluted serum, or 1:4 diluted EDTA-treated plasma were spiked with protein and serially diluted. The diluted samples were assayed and the results were compared with the original spiked sample.

Reproducibility: The inter-assay and intra-assay reproducibility were determined for the BD CBA Human TNF Flex Set by evaluating ten replicates of three different sample levels (inter-assay) and two replicates of three different sample levels from four separate experiments (intra-assay) respectively.