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BD Pharmingen™ PE-Cy™7 Mouse Anti-Human TNF

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BD Pharmingen™ PE-Cy™7 Mouse Anti-Human TNF

品牌： BD Pharmingen

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反应种属：

Human (QC Testing), Rhesus,Cynomolgus,Baboon (Tested in Development)

来源宿主：

Mouse IgG1, κ

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产品介绍

产品信息

耦联标记

PE-Cy7

抗原名称

TNF

宿主

Mouse IgG1, κ

免疫原

Recombinant Human TNF

简单描述

The MAb11 monoclonal antibody specifically binds to human tumor necrosis factor (TNF, also known as TNF-α) protein. TNF is an efficient juxtacrine, paracrine and endocrine mediator of inflammatory and immune functions. It regulates the growth and differentiation of a variety of cell types. TNF is cytotoxic for transformed cells when in conjunction with IFN-γ. It is secreted by activated monocytes/macrophages and other cells such as B cells, T cells and fibroblasts. The immunogen used to generate the MAb11 hybridoma was recombinant human TNF. The MAb11 antibody has been reported to crossreact with Rhesus Macaque TNF.

商品描述

MAb11 The MAb11 monoclonal antibody specifically binds to human tumor necrosis factor (TNF, also known as TNF-α) protein. TNF is an efficient juxtacrine, paracrine and endocrine mediator of inflammatory and immune functions. It regulates the growth and differentiation of a variety of cell types. TNF is cytotoxic for transformed cells when in conjunction with IFN-γ. It is secreted by activated monocytes/macrophages and other cells such as B cells, T cells and fibroblasts. The immunogen used to generate the MAb11 hybridoma was recombinant human TNF. The MAb11 antibody has been reported to crossreact with Rhesus Macaque TNF.

同种型

Mouse IgG1, κ

克隆号

克隆 MAb11 (RUO)

产品详情

PE-Cy7

PE-Cy7 dye is a part of the BD PE family of dyes. This tandem fluorochrome is comprised of a R-Phycoerythrin (PE) donor that has excitation maxima (Ex Max) of 496-nm and 566-nm and an acceptor dye, Cy™7, with an emission maximum (Em Max) at 781-nm. PE can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and detected using an optical filter centered near 781 nm (e.g., a 760/60-nm bandpass filter). The donor dye can be excited by the Blue (488-nm), Green (532-nm) and yellow-green (561-nm) lasers and the acceptor dye can be excited by the Red (627–640-nm) laser resulting in cross-laser excitation and fluorescence spillover. Please ensure that your instrument’s configurations (lasers and optical filters) are appropriate for this dye.

格式:PE-Cy7

激发源:Yellow-Green 561 nm

最多激发源:496 nm, 566 nm

最多发射源:781 nm

应用

实验应用

Intracellular staining (flow cytometry) (Routinely Tested), ELISA (Tested During Development)

推荐用量

5 µl

反应种属

Human (QC Testing), Rhesus,Cynomolgus,Baboon (Tested in Development)

目标/特异性

TNF

背景

别名

Tumor necrosis factor alpha; TNF-a; TNF-α; TNFSF2; Cachectin

制备和贮存

存储溶液

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

保存方式

Aqueous buffered solution containing BSA and ≤0.09% sodium azide.

文献

研发参考(5) 1. Jason J, Larned J. Single-cell cytokine profiles in normal humans: comparison of flow cytometric reagents and stimulation protocols. J Immunol Methods. 1997; 207(1):13-22. (Biology). 2. Prussin C, Metcalfe DD. Detection of intracytoplasmic cytokine using flow cytometry and directly conjugated anti-cytokine antibodies. J Immunol Methods. 1995; 188(1):117-128. (Biology). 3. Sander B, Andersson J, Andersson U. Assessment of cytokines by immunofluorescence and the paraformaldehyde-saponin procedure. Immunol Rev. 1991; 119:65-93. (Biology). 4. Sopper S, Stahl-Hennig C, Demuth M, Johnston IC, Dorries R, ter Meulen V. Lymphocyte subsets and expression of differentiation markers in blood and lymphoid organs of rhesus monkeys. Cytometry. 1997; 29(4):351-362. (Biology). 5. Verdier F, Aujoulat M, Condevaux F, Descotes J. Determination of lymphocyte subsets and cytokine levels in cynomolgus monkeys. Toxicology. 1995; 105(1):81-90. (Biology).

参考图片

Expression of TNF by stimulated human peripheral blood mononuclear cells (PBMC). Human PBMC were stimulated for 4 hrs with PMA (5 ng/ml, Sigma) and Ionomycin (500 ng, Sigma) in the presence of Brefeldin A (Cat. No. 555029). Cells were harvested, fixed, permeabilized and stained with PE mouse anti-human CD8 (Cat. No. 555367) and either PE-Cy7 mouse anti-human TNF antibody (Cat. No.557647/560923/560678, left panel) or PE-Cy™7 Mouse IgG1 κ Isotype Control (Cat. No. 557646, right panel). To demonstrate specificity of staining, the binding of PE-Cy™7 Mouse Anti-Human TNF was blocked by the preincubation of the conjugated antibody with molar excess of recombinant human TNF (0.25 µg,Cat. No. 554618) and the fixed/permeabilized cells with an excess of Purified Mouse Anti-Human TNF (5 µg, Cat. No. 554510) (data not shown) prior to staining. Quadrant markers were set based on the autofluorescence and isotype controls.

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货号：

560923

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