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hTNF RI MAb (Cl 9252 (25 UG)
hTNF RI MAb (Cl 9252 (25 UG)

hTNF RI MAb (Cl 9252 (25 UG)

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品牌： R&D Systems
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    来源宿主：
    Mouse IgG2a
    Mouse IgG2a
    实验应用：
    Western Blot
    Western Blot
    产品介绍
    产品信息
    耦联标记
    Unconjugated
    纯化方式
    Protein A or G purified from ascites
    抗原名称
    CD120a antigen， CD120a， FPF， p55， p55-R， p60， TNF RI， TNFARMGC19588， TNF-R， TNF-R1， TNFR1TBP1， TNFR5
    宿主
    Mouse IgG2a
    免疫原
    E. coli-derived recombinant human TNF RI/TNFRSF1A
    Arg235-Arg455
    Accession # P19438
    克隆号
    925224

    Scientific Data

    Western Blot Detection of Human TNF RI/TNFRSF1A antibody by Western Blot.View Larger

    Detection of Human TNF RI/TNFRSF1A by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB6251) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TNF RI/TNFRSF1A at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

    Human TNF RI/TNFRSF1A Antibody Summary

    Species Reactivity
    Human
    Specificity
    Detects human TNF RI/TNFRSF1A in direct ELISAs.
    Source
    Monoclonal Mouse IgG2A Clone # 925224
    Purification
    Protein A or G purified from ascites
    Immunogen
    E. coli-derived recombinant human TNF RI/TNFRSF1A
    Arg235-Arg455
    Accession # P19438
    Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
    Label
    Unconjugated

    Applications

    Recommended Concentration
    Sample
    Western Blot
    2 µg/mL
    See below

    Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

    应用
    实验应用
    Western Blot
    背景
    别名
    CD120a antigen,CD120a,FPF,p55,p55-R,p60,TNF RI,TNFARMGC19588,TNF-R,TNF-R1,TNFR1TBP1,TNFR55,TNF-R55,TNFR60,TNFRI,TNF-RI,TNF-R-I,TNFR-I,TNFRSF1A,tumor necrosis factor binding protein 1,Tumor necrosis factor receptor 1,tumor necrosis factor receptor 1A isoform beta,tumor necrosis factor receptor superfamily member 1A,tumor necrosis factor receptor superfamily, member 1A,tumor necrosis factor receptor type 1,Tumor necrosis factor receptor type I,tumor necrosis factor-alpha receptor,Tumor Necrosis Factor Receptor I
    背景

    Background: TNF RI/TNFRSF1A

    TNF RI occurs both in membrane bound and soluble forms and functions as a receptor for TNF-alpha and TNF-beta. In the superfamily nomenclature, it is designated TNFRSF1A.

    Long Name
    Tumor Necrosis Factor Receptor I
    Entrez Gene IDs
    7132 (Human); 21937 (Mouse); 25625 (Rat); 102135285 (Cynomolgus Monkey)
    Alternate Names
    CD120a antigen; CD120a; FPF; p55; p55-R; p60; TNF RI; TNFARMGC19588; TNF-R; TNF-R1; TNFR1TBP1; TNFR55; TNF-R55; TNFR60; TNFRI; TNF-RI; TNF-R-I; TNFR-I; TNFRSF1A; tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; tumor necrosis factor receptor 1A isoform beta; tumor necrosis factor receptor superfamily member 1A; tumor necrosis factor receptor superfamily, member 1A; tumor necrosis factor receptor type 1; Tumor necrosis factor receptor type I; tumor necrosis factor-alpha receptor
    研究领域
    制备和贮存
    溶解方法
    Reconstitute at 0.5 mg/mL in sterile PBS.
    保存方式

    Preparation and Storage

    Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
    Loading...
    Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
    Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    参考图片

    Detection of Human TNF RI/TNFRSF1A by Western Blot. Western blot shows lysates of A549 human lung carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human TNF RI/TNFRSF1A Monoclonal Antibody (Catalog # MAB6251) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for TNF RI/TNFRSF1A at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

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    货号：
    MAB6251-SP
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    询价
    25UG
    100UG
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