
产品介绍
产品信息
宿主
鼠

克隆号
3H3

克隆类别
单抗

Product Details
The 3H3 monoclonal antibody reacts with mouse 4-1BB, a TNF receptor superfamily member also known as CD137. 4-1BB is a 39 kDa transmembrane protein expressed by T lymphocytes, NK cells, dendritic cells, granulocytes, and mast cells. Upon binding its ligand 4-1BBL, 4-1BB provides costimulatory signals to both CD4 and CD8 T cells through the activation of NF-κB, c-Jun and p38 downstream pathways. The importance of the 4-1BB pathway has been underscored in a number of diseases, including cancer. Agonistic anti-4-1BB antibodies have been reported to induce T cell mediated antitumor immunity. The 3H3 antibody is an agonistic antibody that has been shown to stimulate 4-1BB signaling in vivo.Specifications
Isotype | Rat IgG2a |
---|---|
Recommended Isotype Control(s) | InVivoPlus rat IgG2a isotype control, anti-trinitrophenol |
Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
Conjugation | This product is unconjugated. Conjugation is available via our Antibody Conjugation Services. |
Immunogen | Mouse CD137 human Fc fusion protein |
Reported Applications | in vivo 4-1BB stimulation in vitro 4-1BB stimulation |
Formulation | PBS, pH 7.0 Contains no stabilizers or preservatives |
Endotoxin* | <1EU/mg (<0.001EU/μg) Determined by LAL gel clotting assay |
Aggregation* | <5% Determined by DLS |
Purity | >95% Determined by SDS-PAGE |
Sterility | 0.2 μM filtered |
Production | Purified from cell culture supernatant in an animal-free facility |
Purification | Protein G |
RRID | AB_2687721 |
Molecular Weight | 150 kDa |
Murine Pathogen Tests* | Ectromelia/Mousepox Virus: Negative Hantavirus: Negative K Virus: Negative Lactate Dehydrogenase-Elevating Virus: Negative Lymphocytic Choriomeningitis virus: Negative Mouse Adenovirus: Negative Mouse Cytomegalovirus: Negative Mouse Hepatitis Virus: Negative Mouse Minute Virus: Negative Mouse Norovirus: Negative Mouse Parvovirus: Negative Mouse Rotavirus: Negative Mycoplasma Pulmonis: Negative Pneumonia Virus of Mice: Negative Polyoma Virus: Negative Reovirus Screen: Negative Sendai Virus: Negative Theiler’s Murine Encephalomyelitis: Negative |
Storage | The antibody solution should be stored at the stock concentration at 4°C. Do not freeze. |
组成成分
肿瘤坏死因子相关跨膜蛋白抗体,磷酸盐缓冲液

应用
反应种属
mouse

背景
研究领域
Cancer Biology, Immune Checkpoint Protein, Immunology
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