首页 试剂盒 ELISA 试剂盒 小分子检测试剂盒
Total NO/NO2-/NO3- S (1 PACK)
Total NO/NO2-/NO3- S (1 PACK)
1/2

Total NO/NO2-/NO3- S (1 PACK)

分享
品牌： R&D Systems
pdf 下载产品说明书
pdf 下载SDS
用小程序，查商品更便捷
收藏
对比对比
咨询咨询
    产品介绍
    产品信息
    产品详情

    Product Summary

    The Parameter Total Nitric Oxide kit has two assay options. Endogenous nitrite is measured in the first option. In the second option, nitrate is converted to nitrite using nitrate reductase and total nitrite is measured. To obtain the nitrate concentration, endogenous nitrite is subtracted from the total nitrite value.

    Recovery

    The recovery of the nitrate standard spiked to levels throughout the range of the assay in various matrices was evaluated.

    Sample TypeAverage % RecoveryRange %
    Cell Culture Supernates (Nitrite Assay) (n=10)10791-115
    Plasma (Nitrate Assay) (n=25)9887-110
    Plasma (Nitrite Assay) (n=20)10492-117
    Serum (Nitrate Assay) (n=10)9589-101
    Serum (Nitrite Assay) (n=10)10998-118
    Urine (Nitrite Assay) (n=10)10187-112

    Linearity

    To assess the linearity of the assay, samples containing and/or spiked with high concentrations of the nitrate standard were serially diluted with the Reaction Diluent to produce samples with values within the dynamic range of the assay.
    Multi-species Total Nitric Oxide and Nitrate/Nitrite ELISA Linearity

    Scientific Data

    N/A Multi-species Total Nitric Oxide and Nitrate/Nitrite ELISA Standard CurveView Larger

    Multi-species Total Nitric Oxide and Nitrate/Nitrite ELISA Standard Curve

    Assay Procedure

    Nitrite Assay Procedure
    Nitrate Reduction Assay Procedure


    Refer to the product for complete assay procedure.

    This assay procedure measures the concentration of endogenous nitrite present in the sample. Bring all reagents and samples to room temperature before use. It is recommended that all samples and standards be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
      3. 50 µL Reaction Diluent (1X)
    3.   Add 50 µL of Reaction Diluent (1X) to the Blank wells.
      4. 50 µL Nitrite Standard or Sample
    4.   Add 50 µL of Nitrite Standard or sample to the remaining wells.
      5. 50 µL Reaction Diluent (1X)
    5.   Add 50 µL of Reaction Diluent (1X) to all wells.
      6. 50 µL Griess Reagent I
    6.   Add 50 µL of Griess Reagent I to all wells.
      7. 50 µL Griess Reagent II
    7.   Add 50 µL of Griess Reagent II to all wells. Mix by gently tapping the side of the plate.
    8.   Incubate at room temperature for 10 minutes. Read at 540 nm with wavelength correction at 690 nm.



    Refer to the product for complete assay procedure.

    This assay procedure measures total nitrite by converting nitrate to nitrite. To determine the nitrate concentration in the sample, the endogenous nitrite concentration measured from the Nitrite Assay Procedure must be subtracted from the converted nitrite concentration measured in this procedure. Bring all reagents and samples to room temperature before use. It is recommended that all samples and standards be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
      3. 50 µL Reaction Diluent (1X)
    3.   Add 50 µL of Reaction Diluent (1X) to the Blank wells.
      4. 50 µL Nitrate Standard or Sample
    4.   Add 50 µL of Nitrate Standard or sample to the remaining wells.
      5. 25 µL NADH
    5.   Add 25 µL of NADH to all wells.
      6. 25 µL Nitrate Reductase
    6.   Add 25 µL of diluted Nitrate Reductase to all wells. Mix well, cover with a plate sealer, and incubate at 37 °C for 30 minutes.
      7. 50 µL Griess Reagent I
    7.   Add 50 µL of Griess Reagent I to all wells.
      8. 50 µL Griess Reagent II
    8.   Add 50 µL of Griess Reagent II to all wells. Mix by gently tapping the side of the plate.
    9.   Incubate at room temperature for 10 minutes. Read at 540 nm with wavelength correction at 690 nm.

    Total Nitric Oxide and Nitrate/Nitrite Parameter Assay Kit Summary

    Format
    96-well strip plate
    Assay Length
    < 1 hour
    Sample Type & Volume Required Per Well
    Cell Culture Supernates (10 uL), Serum (25 uL), EDTA Plasma (25 uL), Heparin Plasma (25 uL), Citrate Plasma (25 uL), Urine (10 uL)
    Sensitivity
    0.78 umol/L
    Assay Range
    3.1 - 200 umol/L (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
    Specificity
    Measures Total Nitric Oxide, Nitrite and Nitrate levels in various samples
    Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
    Interference
    Interference observed with other substances.
    制备和贮存
    保存方式

    Preparation and Storage

    Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
    Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
    声明 ：本官网所有报价均为常温或者蓝冰运输价格，如有产品需要干冰运输，需另外加收干冰运输费。
    货号：
    SKGE001
    一键复制
    询价
    1KIT
    1PACK
    1PACK
    选择数量
    当前规格1件起购 
    配送至
    预计2-3周送达，快递: 免运费，若需干冰额外收费
    询价 大包装询价